Catalogue CPCA-Vim: Chicken Polyclonal Antibody to Vimentin
The Immunogen: Vimentin is the major protein subunit of the 10nm or intermediate filaments protein
found in many kinds of mesenchymal and epithelia cells. Vimentin is also found in many kinds
of cells in tissue culture and in developing neuronal and astrocytic precursor
cells in the central nervous system. Vimentin frequently forms copolymers
with other intermediate filament proteins, such as GFAP (in many kinds
of astrocytes), with desmin (in muscle cells) and neurofilament proteins
(in developing neurons). Antibodies to vimentin are useful in studies
of stem cells and generally to reveal the filamentous cytoskeleton. The
immunogen used to generate our antibody was recombinant human vimentin purified
from E. coli.
Left: Western blot of crude extract of human embryonic kidney Hek293 cells stained with CPCA-Vim, showing a single strong clean band at ~50kDa. Right: View of mixed neuron/glial cultures stained with CPCA-Vim (green) and EnCors rabbit antibody to GFAP antibody (RPCA-GFAP, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Antibody Characteristics: This antibody was generated in chicken by
standard procedures and immunoglobulin was extracted from egg yolk. The resulting
polyclonal antibody belongs to the IgY subclass. This is the chicken homologue
of mammalian IgG and can be used in the same general way, with the caveat that
this type of antibody does not bind either Protein A or Protein G. Suitable
second antibody reagents can be obtained from many vendors including Molecular
Probes and Sigma-Aldrich. Store at 4°C or -20°C. Avoid repeat freezing and thawing.
Suggestions for use: The IgY preparation has a concentration of ~20mg/ml. The titre of this material is about 1:5,000 in immunofluorescence microscopy.
Examples: For some other on line images generated with this antibody check here
Price and Availability: - We currently supply 100 microliter aliquots for $200. Material is in stock and ready for immediate shipping.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
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