Catalogue# MCA-1D2: Alpha-Internexin/NF66 Monoclonal Antibody 1D2-INA
The Immunogen:a-internexin is a Class IV intermediate filament subunit. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H, and a-internexin is the major neurofilament protein of developing neurons. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The HGNC name for this protein is INA.
We are OEM suppliers of this antibody- in other words we manufactured it, characterized it and generated the data presented on this page. This antibody is available from several other vendors, but we can supply it more cheaply and we can provide you with more detailed information on the properties of the antibody.
Left: Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with MCA-1D2. MCA-1D2 stains a band of about 66kDa in all three preparations. Right: Mixed cultures of rat CNS cells stained with MCA-1D2 (red) and EnCor's chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody CPCA-MAP2). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.
Antibody characteristics: MCA-1D2 was raised against purified recombinant rat a-internexin expressed in and purified from E. coli. MCA-1D2 reacts with human a-internexin and with this protein in all mammalian species tested to date. Clean and specific on immunoblots. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the 1D2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections. In most respects this antibody is quite comparable to MCA-2E3, also available from EnCor.
Suggestions for use: For immunofluorescence try dilutions of 1:500, for immunohistochemistry with ABC or other enzymatic amplification procedures try 1:5,000. For western blots try 1:10,000 dilutions.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
References:
1. Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, Shaw G. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 2002.OMIM link: Press here
Availability and Price: Available for shipping now, $200 US per aliquot of 500 µLs of concentrated tissue culture supernatant material, enough for hundreds of experiments. For order form press here,
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