Raised against human c-Fos, validated on stimulated neurons and HeLa cells and which work well on histological sections.
Above: Adult rat hippocampus was treated with mouse monoclonal antibody to c-Fos MCA-2H2 in red, rabbit polyclonal antibody to NeuN/Fox3 RPCA-Fox3 in green and the DNA intercallating dye DAPI in blue. A few spontaneously active neurons appear yellow (arrowed) as they express both c-Fos and NeuN/Fox3. Our affinity purified rabbit antibody RPCA-c-Fos-ap will work in exactly the same way.
The c-Fos protein is a member of Fos family of transcription factors and is a cellular counterpart of the retroviral oncogene v-Fos. The v indicates a mutated and oncogenic viral variant and c indicates the normal cellular form. Others member of Fos family are FosB, Fra-1 and Fra-2. Fos proteins associate with c-Jun proteins, but also with other basic leucine zipper (bZIP) domain proteins to create a variety of AP-1 (activator protein-1) complexes which bind to the promoters of numerous other genes and induce their expression. Dimeric complex of these AP-1 transcription factors as a result generally rapidly activate physiological processes such as cell proliferation, differentiation, neoplastic transformation, apoptosis, and response to stress. Expression of c-Fos protein is normally low but is induced rapidly and transiently in response to a wide array of stimuli including serum, growth factors, tumor promoters, cytokines, UV radiation and, in neurons, electrical activation. In fact several lines of data demonstrate that expression of c-Fos by individual neurons can be used as a marker of cell activation, as there is an association between c-Fos expression and the firing of action potentials. Such work is usually done on histological sections of brain tissue and can be used to trace neuroanatomical connections, identify seizure pathways and the sites of action of neuroactive drugs. Our antibodies works very well in this kind of preparation.
|Above: Rat brain neural cultures (left) and the same cells stimulated with membrane depolarization buffer for 5 hours (right). This is a salt solution containing 170mM Potassium which depolarizes and stimulates gene expression in neuronal cells but has no effect on glia. Cultures were stained with our monoclonal antibody to c-Fos, MCA-2H2 in green and rabbit anti-GFAP, RPCA-GFAP in red. Nuclear DNA is revealed the DNA intercalating dye DAPI in blue. Click on each image for a larger view. The c-Fos expression is strongly induced only in stimulated neuronal cells and localizes in the nucleus, while unstimulated neurons and glia show no c-Fos staining. The c-Fos antibody was used at a dilution of 1:1,000 from a 1mg/mL solution. The GFAP antibody was used at a dilution of 1:1,000 from a serum preparation. Cultures were processed using our standard fixation and staining procedure (described here). To order this antibody go to our order form (here) or check out from the antibody page here. Picture taken with a Zeiss 40X objective and documented with a SPOT camera. Mouse click on each image to get an enlarged view. Our affinity purified rabbit antibody RPCA-c-Fos-ap will work in exactly the same way, see here.|
|Above: HeLa cells were serum-starved (Left) or serum-starved and then stimulated with 20% fetal bovine serum (FBS) for 2 hours (Right). The were then stained under identical conditions with our monoclonal antibody to c-Fos, MCA-2H2 (green) and our chicken anti-vimentin (CPCA-Vim, red). Nuclear DNA was revealed with DAPI (blue). Click on each image for a larger view. Serum starvation inhibits c-Fos expression, while treatment with FBS for 2 hours strongly stimulates c-Fos expression which localizes in the nucleus as shown. The c-Fos antibody was used at a dilution of 1:1,000 from a 1mg/mL solution. The vimentin antibody was used at a dilution of 1:100,000. Cultures were processed using our standard fixation and staining procedure (described here). To order the c-Fos antibody go to our order form (here) or use our online store here. Picture taken with a Zeiss 40X objective and documented with a SPOT camera. Mouse click on each image to get an enlarged view. Our affinity purified rabbit antibody RPCA-c-Fos-ap will work in exactly the same way, see here|
Our mouse monoclonal antibody MCA-2H2 and our affinity purified rabbit polyclonal antibody RPCA-c-Fos-AP were generated against full length human recombinant c-Fos protein expressed in and purified from E. coli. This recombinant form is also available from EnCor, see Prot-r-c-Fos. The HGNC name for this protein is FOS.