HSP27, chicken polyclonal, Cat# CPCA-HSP27

HSP27, chicken polyclonal, Cat# CPCA-HSP27

HeLa cells stained with CPCA-HSP27 (green), and counterstained with monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) MCA-1F3 and DNA (blue). CPCA-HSP27 antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.

Blot of HeLa cell crude extracts blotted with CPCA-HSP27. Note the strong clean bands at 27kDa corresponding to HSP27.

Product name Anti-Heat Shock Protein 27, HSP27
Description Chicken Polyclonal to Anti-Heat Shock Protein 27
Reference Code CPCA-HSP27
HGNC name HSBP1
RRID# AB_2572328
Molecular weight 27 kDa
Immunogen Recombinant full length purified HSP27 from E. coli
Isotype IgY
Concentration Antibody is supplied as an aliquot of concentrated IgY preparation.
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blot: 1:5,000. IF/ICC and IHC:1:2,000 .
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, which are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an an apparent molecular weight of 27kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. The HGNC name for this protein is HSBP1.

References:
1. Wyttenbach, A et al. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11:1137-1151 (2002).
2. Evgrafov, OV et al. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36:602-606 (2004).

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