Hsp27, chicken polyclonal, Cat# CPCA-Hsp27
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HeLa cells stained with CPCA-HSP27 (green), and counterstained with EnCor’s monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) MCA-1F3 and DNA (blue). CPCA-HSP27 antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.
Blot of HeLa cell crude extracts blotted with CPCA-HSP27. Note the strong clean bands at 27kDa corresponding to HSP27.
Anti-Heat Shock Protein 27, HSP27
Chicken Polyclonal to Anti-Heat Shock Protein 27
Recombinant full length purified HSP27 from E. coli
Antibody is supplied as an aliquot of concentrated IgY preparation.
Human, horse, cow, pig, chicken, rat, mouse
Western blot, ICC/IF, IHC
Suggestions for use
Western blot: 1:5,000. IF/ICC and IHC:1:2,000 .
Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
heat shock proteins
were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, which are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the
heat shock factor
1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an an apparent molecular weight of 27kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases,
Charcot-Marie-Tooth disease type 2F
distal hereditary motor neuropathy IIB
(2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. The
name for this protein is
1. Wyttenbach, A et al. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Hum. Molec. Genet. 11:1137-1151 (2002)
2. Evgrafov, OV et al. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Nature Genet. 36:602-606 (2004).
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