Myelin Basic Protein, MBP, chicken polyclonal, Cat# CPCA-MBP

Myelin Basic Protein, MBP, chicken polyclonal, Cat# CPCA-MBP



Rat mixed neuron/glial cultures stained with chicken Myelin Basic Protein (red). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for myelin basic protein. Astrocytes are stained, in green with MCA-5C10, our monoclonal antibody to glial fibrillary acidic protein (GFAP), and excellent marker of astrocytes. specifically, and that the staining is concentrated in the cell bodies.

Blots of whole tissue homogenate of rat spinal cord probed with CPCA-MBP, chicken antibody to Myelin Basic Protein, in the first two lanes. The antibody stains 21.5 kDa, 18.5kDa, 17kDa and 14kDa, all four CNS isotypes of rat myelin basic protein. Our two monoclonals to MBP, MCA-7G7 and MCA-7D2, show similar staining in the lanes indicated, although MCA-7D2 shows stronger binding for the 21.5 kDa and 18.5 kDa isotypes.

Product name Anti-MBP
Description Chicken Polyclonal to Anti-Myelin Basic Protein, MBP
Reference Code CPCA-MBP
HGNC name MBP
RRID# AB_2572352
Molecular weight 14, 17, 18.5, and 21.5 kDa
Immunogen Purified myelin basic protein isolated from cow nerve.
Isotype IgY
Concentration Antibody is supplied as an aliquot of IgY preparation.
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, IF/ICC, IHC
Suggestions for use Western blot: 1:10,000. IF/ICC and IHC: 1:1,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In this case the proteins are referred to as Golli-MBP proteins and result from the use of 3 exons not utilized in the nervous system, resulting in proteins with unique N-terminal regions.

In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 20.5, 18.5, and 17.2 kDa in humans. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14 kDa. Sequence alignments of both human and rodent isotypes can be downloaded from here. We have found that our chicken antibody stains all four transcripts on blots with equivalent intensity. We also market two good mouse monoclonal antibodies to MBP, MCA-7G7 and MCA-7D2. The HGNC name for this protein is MBP.

Antibody was raised in chicken against purified myelin basic protein isolated from cow nerve. This antibody is an IgY preparation, with total protein content about 30 mg/mL.


References:

1. Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. Basic A1 protein of the myelin membrane. The complete amino acid sequence. J. Biol. Chem. 246:5770-5784 (1971).

2. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861 (2002).

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