View of mixed neuron/glial cultures stained with CPCA-Vim (green) and EnCor rabbit antibody to GFAP, RPCA-GFAP,
(in red). Vimentin is the sole cytoplasmic intermediate filament subunit expressed in fibroblasts, microglial and endothelial cells. The flattened cells in the middle of the image which appear green are fibroblasts. Astrocytes may express primarily GFAP, or both GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
||Western blot of Rat whole brain extract, HeLa, SH-SY5Y, HEK293, and NIH/3T3 cells probed with CPCA-Vim (red), showing a single strong band at ~50 kDa. Blot was simultaneously probed with another EnCor antibody against MAP2 (green), MCA-2C4.
||Chicken Polyclonal to Anti-Vimentin
||Recombinant human vimentin expressed in and purified from E. coli.
||Antibody is supplied as an aliquot of concentrated IgY prep.
||Western blot, ICC/IF, IHC
|Suggestions for use
||Western blot: 1:10,000. ICC/IF and IHC: 1:5,000.
||Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid repeated freeze/thaw cycles.
Vimentin is 10nm subunit of intermediate filaments
, which are a major component of the cytoskeleton
in most cell types. Vimentin is found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke
and Klaus Weber
(1). The name derives from the Latin “Vimentum
“, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts
, and in developing neuronal and astrocytic precursor cells in the central nervous system.
Many cell lines such as HEK293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient (2).
Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate this antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce two monoclonal antibodies to vimentin MCA-2A52 and MCA-2D1. The HGNC name for this protein is VIM.
1. Franke, W. W., Schmid, E., Osborn, M. and Weber, K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc. Natl. Acad. Sci. USA 75:5034–5038 (1978).
2. Muller, M., Bhattacharya, S. S., Moore, T., Prescott, Q., Wedig, T., Herrmann, H., Magin, T. M. Dominant cataract formation in association with a vimentin assembly disrupting mutation. Hum. Molec. Genet. 18:1052-1057 (2009).
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