Microtubule Associated Protein 2, MAP2C/D, mouse monoclonal, Cat# MCA-2C4

Microtubule Associated Protein 2, MAP2C/D, mouse monoclonal, Cat# MCA-2C4



Mixed neuron and glia cultures stained with MCA-2C4 (red) and chicken antibody to Vimentin (green) and DNA (blue). MCA-2C4 antibody reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while vimentin was visualized in astrocytes and fibroblasts.

Western blot of Rat whole brain extract, HeLa, SH-SY5Y, HEK293, and NIH/3T3 cells probed with mouse monoclonal antibody MCA-2C4, raised against recombinant human MAP2D (green). All forms of MAP2 are present in the rat brain homogenate and are recognized by this antibody. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. Bands appear at about 70kDa corresponding to MAP2C/D isotypes. The fainter bands in between likely correspond to in Vivo fragments of MAP2A/B, since both proteins are very sensitive to proteases. Of the four cell lines tested, only the SH-SY5Y cell line, which has neuronal properties, is positive for ~70kDa MAP2C/D isotypes. The blot was simultaneously probed with our chicken polyclonal antibody against vimentin, CPCA-Vim (red), showing a single strong band at ~50 kDa, which is present in all preparations.

Product name Anti-MAP2A/B/C/D
Description Mouse Monoclonal to Anti-Microtubule Associated Protein 2, MAP2C/D
Reference Code MCA-2C4
HGNC name MAP2
RRID# AB_2572215
Molecular weight MAP2A/B ~2*0 kDa, MAP2C/D ~70kDa by SDS-PAGE
Immunogen Full length recombinant human MAP2D protein.
Isotype IgG1
Concentration Antibody is supplied as an aliquot of 1 mg/mL of affinity purified antibody.
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blot: 1:5,000-1:10,000. ICC/IF and IHC: 1:5,000-10,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2A and MAP2B.

A third lower molecular weight form, usually called MAP2C and MAP2D, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All four MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (a.k.a. MAP-tau or tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers of neuronal cells, their perikarya and neuronal dendrites. MCA-2C4 was raised against recombinant human MAP2D and also binds to recombinant human MAP2C. The antibody epitope is therefore in the core of the MAP2 molecule and so this antibody will bind all known MAP2 isoforms as shown in the blot. MAP2C and MAP2D are expressed earlier in development than the MAP2a and MAP2b isoforms, so that this antibody is a more useful marker of neuronal development. In contrast MAP-τ (MAP-tau) is found predominantly but not exclusively in neuronal axons. The HGNC name for this protein is MAP2.


References:

1. Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 6:204 (2004)

2. Vallee R. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J. Cell Biol. 92:435-442 (1992)

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