ALDH1L1, mouse monoclonal, Cat# MCA-2E7

ALDH1L1, mouse monoclonal, Cat# MCA-2E7

Neuron-glia cell mixed cultures stained with MCA-2E7 (red) and our chicken polyclonal antibody against vimentin: CPCA-Vim (green). Blue is a DNA stain. MCA-2E7 stains astrocytic cell bodies and processes. The fibroblastic cells contain only vimentin and so are green, while astrocytes contain either vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.

Blot of rat liver tissue homogenates blotted with MCA-2E7. MCA-2E7 binds strongly a band at about 100 kDa.

Product name Antibody to Aldehyde Dehydrogenase H1L1
Description Mouse monoclonal to Aldehyde Dehydrogenase H1L1
Reference Code MCA-2E7
HGNC name ALDH1L1
RRID# AB_2572220
Molecular weight 100 kDa
Immunogen Amino acids 402-902 of human ALDH1L1 expressed in and purified from E. coli.
Concentration Antibody is supplied as an aliquot of 1 mg/mL of affinity purified antibody or concentrated tissue culture supernatant.
Isotype IgG1
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blots: 1:5,000-1:10,000. IF/IHC: 1:2,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Aldehyde dehydrogenases (ALDH) are a group of enzymes that catalyze oxidation (dehydrogenation) of aldehydes. To date, nineteen ALDH genes have been identified within the human genome. They belong to 15 families in ALDH superfamily. Among these, aldehyde dehydrogenase family 1, member 1 (ALDH1L1) catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 expression is tissue-specific and is highly expressed in the liver, representing up to 1% of the total pool of soluble cell proteins in the mammalian liver (1).

In an earlier study, Cahoy et al. applied FACS (Fluorescent-Activated Cell Sorting) to isolate astrocytes from EGFP (Enhanced Green Fluorescent Protein) transgenic-mouse, then created a transcriptome database of the expression levels of 20,000 genes by gene profiling of neurons, astrocytes and oligodendrocytes using Affymetrix GeneChip Arrays (2). They identified ALDH1L1 as a highly and specifically expressed gene in astrocytes. ALDH1L1 is more widely expressed throughout the brains, while astrocyte marker GFAP shows more predominant expression in white matter. In addition, loss of function or expression of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression, suggesting its role as a biomarker and a target in cancer therapy (3,4,5).

Monoclonal antibody MCA-2E7 was raised against amino acids 402-902 of human ALDH1L1 expressed in and purified from E. coli. The HGNC name for this protein is ALDH1L1.


References:

1. Kisliuk RL. Folate biochemistry in relation to antifolate selectivity. In:Jackman AL, editor. Antifolate drugs in cancer therapy. Totowa, NJ: Humana Press; p. 13-36 (1999). ISBN 0896035964.

2. Cahoy JD, Emery B, Kaushal A, et al. A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci.28:264-78 (2008).

3. Krupenko SA, Oleinik NV. 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells. Cell Growth Differ.13:227-36 (2002).

4. Rodriguez FJ, Giannini C, Asmann YW, et al. Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. J Neuropathol Exp Neurol. 67:1194-204 (2008).

5.Oleinik NV, Krupenko NI, Krupenko SA. Epigenetic Silencing of ALDH1L1, a Metabolic Regulator of Cellular Proliferation, in Cancers. Genes Cancer. 2:130-9 (2011).

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