Immunofluorescent analysis of Rat mixed neuron/glial cell cultures stained with Mouse mAb against 14-3-3η (MCA-3G12, red). Neuronal perikarya are very rich in 14-3-3η which has a diffused cytoplasmic staining pattern. The blue is DAPI staining of nuclear DNA.
Western blot analysis of neural tissue and cell lysates using Mouse mAb against 14-3-3η (MCA-3G12, green).  protein standard,  Rat whole brain,  Mouse whole brain,  NIH/3T3,  Hek293,  HeLa,  SH-SY5Y,  C6 cells.
||Mouse Monoclonal Antibody to 14.3.3η (eta)
||Full length human recombinant human protein
||Supplied as an aliquot of affinity purified antibody at 1mg/mL in PBS, 50% glycerol, 5mM NaN3
||Human, cow, pig, chicken, rat, mouse
||Western blot, ICC/IF, IHC
|Suggestions for use
||WB: 1:2,000. IF/IHC: 1:500-1:1,000.
||Store at 4°C for short term and -20°C for long term. Avoid repeated freeze / thaw cycles.
The 14.3.3 family of proteins was originally discovered as prominent protein spots on 2-dimensional gels. They are a family of 28-33 kDa proteins which make up the major portion of cytoplasm proteins (1). They act as binding partners for phosphoserine and phosphothreonine sites in other proteins, though they also have binding partners which are not phosphorylation-dependent. These binding interactions are important in the regulation of molecules such as signaling kinases in the MAP kinase pathway
, c-Raf and b-Raf, the proapoptotic molecules Bad and Bax, and the cell cycle regulator Cdc25. There are 7 mammalian 14.3.3 proteins and they are normally expressed as homo- or in some cases hetero-dimers. 14.3.3η or 14.3.3 eta is widely expressed and concentrated in the nervous system. The HGNC name for 14.3.3 is YWHAH, and an alternate name is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 1, due to this protein’s role in activation of tyrosine and tryptophan hydroxylases. The 14-3-3η protein accumulates in the CSF of patients suffering from Creutzfeld-Jacob Disease, and thus can be used for the diagnosis of this disease (2). Furthermore, this protein binds α-synuclein in the Lewy bodies of Parkinson’s disease-affected brains and has been linked to early-onset schizophrenia (3,4).
1. Morrison, DK. The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development. Trends Cell Biol. 19:16-23 (2009).
2. Hsich G, Kenney K, Gibbs CJ, Lee KH and Harrington MG. The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. N Engl J Med. 335:924-30 (1996).
3: Ubl A, Berg D, Holzmann C, Krüger R, Berger K, Arzberger T, Bornemann A, Riess O. 14-3-3 protein is a component of Lewy bodies in Parkinson’s disease-mutation analysis and association studies of 14-3-3 eta. Brain Res Mol Brain Res. 108(1-2):33-9 (2002).
4: Middleton FA, Peng L, Lewis DA, Levitt P, Mirnics K. Altered expression of 14-3-3 genes in the prefrontal cortex of subjects with schizophrenia. Neuropsychopharmacology 30(5):974-83 (2005).
Download Adobe Acrobat format product data sheet: .