Myelin Basic Protein, MBP, mouse monoclonal, Cat# MCA-7G7

Myelin Basic Protein, MBP, mouse monoclonal, Cat# MCA-7G7



Rat mixed neuron/glial cultures stained with Myelin Basic Protein monoclonal antibody MCA-7G7 (red). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.

Blots of 20µg crude rat brain homogenate blotted with 2 MBP antibodies;  MCA-7G7 (lane 1) at 1:5000 and MCA-7D2 (lane 2) at 1:5000 dilution. MCA-7G7  bind all four transcripts: 21.5kDa,18.5kDa, 17kDa and 14kDa , while MCA-7D2 monoclonal binds the largest 21.5 kDa and 18.5 kDa transcripts preferentially.

Product name Anti-MBP
Description Mouse Monoclonal to Anti-Myelin Basic Protein, MBP
Reference Code MCA-7G7
HGNC name MBP
RRID# AB_2572353
Molecular weight 14, 17, 18.5, and 21.5 kDa in rodent, 17.2, 18.5, 20.5 and 21.5 in human
Immunogen Purified myelin basic protein isolated from cow nerve.
Isotype IgG1
Concentration Antibody is supplied as an aliquot of 1mg/mL of affinity purified antibody or ascites fluid.
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, IF/ICC, IHC
Suggestions for use Western blot:1:5,000 -1:10,000. IF/ICC and IHC: 1:1,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In this case the proteins are referred to as Golli-MBP proteins and result from the use of 3 exons not utilized in the nervous system, resulting in proteins with unique N-terminal regions.

In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, the protein products having molecular weights of 21.5, 20.5, 18.5, and 17.2 kDa in humans. The mammalian gene has 7 exons, and in humans exons 2 and 5 are either included or not, producing these four proteins. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14 kDa. In this case the homologous exons are utilized differently: exons 2 and 6 are either inserted or not. Sequence alignments of both human and rodent isotypes can be downloaded from here. We have found that MCA-7G7 and our chicken antibody, CPCA-MBP, stain all four transcripts on blots, whereas our mouse monoclonal MCA-7D2 binds only the 21.5 kDa and 18.5 kDa isotypes, so that epitope is dependent of protein sequence encoded by exon 2. The HGNC name for this protein is MBP.


References:

1. Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. Basic A1 protein of the myelin membrane. The complete amino acid sequence. J. Biol. Chem. 246:5770-5784 (1971).

2. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861 (2002).

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