Poster #16

Poster #16


Adult rat cerebellum stained with CPCA-MBP, a chicken polyclonal antibody to the myelin basic protein in green and MCA-1B11, a mouse monoclonal antibody to neurofilament NF-L in red. Nuclei revealed with DAPI DNA stain in blue. 13.4 megapixel image, will print 24” X 36” at 125 DPI. To see a larger version of this image, as part of a slide show of our other images, go here.

Image was taken on a Olympus FV1000 confocal microscope fitted with a programmable stage which allows a series of images to be taken and then merged into one large image.

This image was made with our chicken polyclonal antibody to mylein basic protein (MBP), CPCA-MBP in green and our mouse monoclonal to neurofilament light chain (NF-L) MCA-1B11 in red. Blue is the DNA intercalating agent DAPI, which is in the Vestashield mounting medium we normally use (Vector labs Cat#1200). Sections were cut at 25μM on a vibratome after perfusion and fixation in paraformaldehyde as described here.

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