Poster #18

Poster #18


Adult rat brain stem nuclei stained with mouse monoclonal antibody to NeuN/Fox3, MCA-1B7, in green and CPCA-MAP2, a chicken polyclonal antibody to MAP2 in red. DNA in nuclei revealed with DAPI 22.2 megapixel image, will print 24” X 36” at 160 DPI. To see a larger version of this image, as part of a slide show of our other images, go here.

Image was taken on a Olympus FV1000 confocal microscope fitted with a programmable stage which allows a series of images to be taken and then merged into one large image.

This image was made with our chicken polyclonal antibody to MAP2, CPCA-MAP2 in red and our mouse monoclonal to NeuN/Fox3 MCA-1B7 in red. Blue is the DNA intercalating agent DAPI, which is in the Vestashield mounting medium we normally use (Vector labs Cat#1200). Section was cut at 25μM on a vibratome after perfusion and fixation using our standard procedure outlined here.

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