Poster #20

Poster #20


Adult rat brain stem nuclei stained with NeuN/Fox3 antibody in red (CPCA-Fox3) and mouse monoclonal antibody to MAP2 MCA-5H11 in green. Dendrites of neurons are visible with the MAP2 stain, while the perikarya are positive for NeuN/Fox3. Nuclei of cells are visualized in blue with the DAPI DNA stain. 21.2 megapixel image, print is 24” X 36” at 156 DPI on highest quality lustre paper. To see a larger version of this image, as part of a slide show of our other images, go here.

Image was taken on a Olympus FV1000 confocal microscope fitted with a programmable stage which allows a series of images to be taken and then merged into one large image.

Adult rat brain stem stained with RPCA-FOX3, our rabbit polyclonal antibody to NeuN/Fox3 in red and MCA-5H11, a mouse monoclonal antibody to MAP2 in green. 21.2 megapixel image, will print 24” X 36” at 156 DPI. using our standard procedure outlined here. Section was cut at 25μM on a vibratome after perfusion and fixation using our standard procedure outlined here.

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