Poster #05

Poster #05


Poster #5
Mixed E20 rat cortical cultures stained with monoclonal antibodyto vimentin MCA-2A52 and chicken polyclonal antibody CPCA-GFAP to glial fibrillary acidic protein (GFAP). Nuclei of neurons and glia are seen in blue with the DNA stain DAPI. 43.6 megapixel image, print is 24” X 36” at 248 DPI. To see a larger version of this image go here.

Image was taken on a Olympus FV1000 confocal microscope fitted with a programmable stage which allows a series of images to be taken and then merged into one large image.

This image was made with our chicken polyclonal antibody to GFAP, CPCA-GFAP in green and our mouse monoclonal to vimentin MCA-2A52 in red. Blue is the DNA intercalating agent DAPI, which is in the Vestashield mounting medium we normally use (Vector labs Cat#1200). Cells were derived from E20 rat brain and were processed using our standard procedure outlined here.

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