Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name (1). Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in rat pheochromacytoma PC12 cells (2,3). Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig’s disease (Amyotrophic Lateral Sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration
Coomassie brilliant blue stained SDS-PAGE gel of various recombinant proteins. His-tagged human Peripherin, was expressed and purified from E. coli
BL21 using immobilized metal affinity chromatography. 1 mg of protein was run on each lane, and the lane indicated with “Peri” contains the Peripherin protein. The other lanes show recombinant His-tagged α-Internexin, Vimentin and NF-L as indicated. Protein molecular weight standards are in the first lane and apparent molecular weights are as indicated.
A cDNA encoding full length human Peripherin was inserted into a eukaryotic expression vector which adds an N-terminal in frame His-tag. This was transformed into E. coli and recombinant protein was purified in 6M urea using immobilized metal affinity chromatography. Purified protein was diluted to 1 mg/mL and is supplied in 6M urea.
1. Portier MM, de Néchaud B, Gros F. Peripherin, a new member of the intermediate filament protein family. Dev Neurosci. 6:335-44 (1984).
2. Troy CM, Brown K, Greene LA, Shelanski ML. Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo. Neuroscience. 36:217-37 (1990).
3. Aletta JM, Angeletti R, Liem RK, Purcell C, Shelanski ML, Greene LA. Relationship between the nerve growth factor-regulated clone 73 gene product and the 58-kilodalton neuronal intermediate filament protein (peripherin).J Neurochem. 51:1317-20 (1988).
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