Left: Mixed neuron-glial cell mixture cultures stained with RPCA-ALDH1L1 (red) and our monoclonal antibody against GFAP: MCA-5C10 (green). RPCA-ALDH1L1 stains astrocytes and excludes from neuron cells. ALDH1L1 stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes. Astrocytes are positive for both ALDH1L1 and GFAP appear yellow. ALDH1L1 also labels many astrocytes not labelled by GFAP, which appear as red. Right: Mouse brain sections (fixed by transcardial perfusion of 4% paraformaldehyde) stained with RPCA-ALDH1L1 antibody (green) and MCA-5C10 (red). Along the corpus callosum (white matter), RPCA-ALDH1L1 labels astrocytes that also heavily express GFAP. As a result, astrocytes in this area appear to be gold to yellow. Inset shows a magnified image of RPCA-ALDH1L1 staining. Nuclei are labeled with Dapi (blue).
|Blot of rat liver tissue homogenates blotted with RPCA-ALDH1L1. RPCA-ALDH1L1 binds strongly a band at ~100 kDa.
||Anti-Aldehyde Dehydrogenase H1L1
||Rabbit Polyclonal to anti-Aldehyde Dehydrogenase H1L1
||Full length human protein
||Antibody is supplied as an aliquot of serum.
||Human, horse, cow, pig, chicken, rat, mouse
||Western blot, ICC/IF, IHC
|Suggestions for use
||Western blots: 1:5,000-1:10,000. IF/IHC: 1:2,000.
||Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Aldehyde dehydrogenases (ALDH) are a group of enzymes that catalyze oxidation (dehydrogenation) of aldehydes. To date, nineteen ALDH genes have been identified within the human genome. They belong to 15 families in ALDH superfamily. Among these, aldehyde dehydrogenase family 1, member 1 (ALDH1L1) catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 expression is tissue-specific and is highly expressed in the liver, representing up to 1% of the total pool of soluble cell proteins in the mammalian liver (1).
In an earlier study, Cahoy et al. applied FACS (Fluorescent-Activated Cell Sorting) to isolate astrocytes from EGFP (Enhanced Green Fluorescent Protein) transgenic-mouse, then created a transcriptome database of the expression levels of 20,000 genes by gene profiling of neurons, astrocytes and oligodendrocytes using Affymetrix GeneChip Arrays (2). They identified ALDH1L1 as a highly and specifically expressed gene in astrocytes. ALDH1L1 is more widely expressed throughout the brains, while astrocyte marker GFAP shows more predominant expression in white matter. In addition, loss of function or expression of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression, suggesting its role as a biomarker and a target in cancer therapy (3,4,5).
Polyclonal antibody RPCA-ALDH1L1 was raised against full length recombinant human ALDH1L1 expressed and purified from E. coli. The HGNC name for this protein is ALDH1L1.
1. Kisliuk RL. Folate biochemistry in relation to antifolate selectivity. In:Jackman AL, editor. Antifolate drugs in cancer therapy. Totowa, NJ: Humana Press; p. 13-36 (1999). ISBN 0896035964.
2. Cahoy JD, Emery B, Kaushal A, et al. A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci.28:264-78 (2008).
3. Krupenko SA, Oleinik NV. 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells. Cell Growth Differ.13:227-36 (2002).
4. Rodriguez FJ, Giannini C, Asmann YW, et al. Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. J Neuropathol Exp Neurol. 67:1194-204 (2008).
5．Oleinik NV, Krupenko NI, Krupenko SA. Epigenetic Silencing of ALDH1L1, a Metabolic Regulator of Cellular Proliferation, in Cancers. Genes Cancer. 2:130-9 (2011).
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