Green Fluorescent protein FP506 , rabbit polyclonal, Cat# RPCA-FP506
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Transfected HEK293 cells which overexpress FP506 protein were stained with RPCA-FP506 and viewed in the fluorescence microscope. HEK293 Cells which are transfected with FP506 (left panel) are bright green. On staining with RPCA-FP506 in red (middle panel), cells appear orange (right panel). Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain.
Blot of HEK293 cells transfected with pFin-EF1-FP506 vector (lane 1) and non-transfected HEK293 (lane 2) was probed with RPCA-FP506. There is a strong clean band at ~27 kDa corresponding to FP506 in FP506 transfected HEK 293 cells, but not in non-transfected cells.
Rabbit Polyclonal Antibody to FP506
Full length recombinant protein based on the FP506 sequence in Matz et al. 1999
Antibody is provided as crude serum.
Western blot, ICC/IF, IHC
Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Suggestions for use
Western blot: 1:1,000-5,000
Fluorescent proteins have become widely used in a variety of experimental paradigms since the demonstration of the utility of the first one, Green Fluorescent Protein (GFP) about 20 years ago (1). In 1999 Matz et al. characterized several fluorescent proteins from various coral species which were cloned and sequenced using degenerate primers based on the GFP sequence (2). On of these was FP506, isolated from a
Zoanthus coral. This protein proved to have spectral properties similar to GFP although the protein sequence was only distantly related.
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression.
Science 263:802-5 (1994).
2. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML and Lukyanov SA. Fluorescent proteins from nonbioluminescent Anthozoa species
Nat. Biotechnol. 17:969-973 (1999).
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