GAP43, rabbit polyclonal, Cat# RPCA-GAP43

GAP43, rabbit polyclonal, Cat# RPCA-GAP43

Mixed neuron-glial cultures stained with RPCA-GAP43 (red). Blue is DNA staining.

Western blots of homogenate of cow cerebellum stained with RPCA-GAP43 . A prominent band running at ~43 kDa represents the full length GAP43.

Product name RPCA-GAP43
Description Rabbit polyclonal to GAP43
Reference Code RPCA-GAP43
HGNC name GAP43
RRID# AB_2572288
Molecular weight 43 kDa on SDS-PAGE, actually 23-26 kDa
Immunogen C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein
Concentration Antibody is supplied as an aliquot of affinity purified antibody
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blots: 1:10,000
IF/ICC and IHC: 1:1,000

GAP43 is a very abundant nervous system protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1).

Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case, the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule.

Mammalian GAP43 protein contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack a well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

References:

1. Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells.J. Cell Biol. 89:86-95 (1981).

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