MARCKS, rabbit polyclonal, Cat# RPCA-MARCKS

MARCKS, rabbit polyclonal, Cat# RPCA-MARCKS


View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and CPCA-MAP2, EnCor’s chicken polyclonal antibody to MAP2. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.

Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with RPCA-MARCKS, at 1:10,000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~80 kDa corresponds to MARCKS.

Product Name Anti-MARCKS
Description Rabbit Polyclonal to MARCKS (Myristoylated alanine rich C Kinase substrate)
Reference Code RPCA-MARCKS
HGNC name MARCKS
RRID# AB_2250333
Molecular weight 80 kDa
Immunogen Full length recombinant human MARCKS purified from E. coli
Isotype IgG
Concentration Antibody is supplied as an aliquot of serum
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Suggestions for use Western blot: 1:10,000-1:20,000. IF/ICC and IHC: 1:500-1:1,000. ABC: 1:5,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells (1). The MARCKS protein runs at about 80 kDa on SDS-PAGE gels, although the real molecular is much lower, at about 31.5 kDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less to SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. MARCKS is a major protein of the brain, and is concentrated in the synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind to both actin and calmodulin in vitro (2). MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties (3). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (4).
The HGNC name for this protein is MARCKS.

References:

1. Hirai, M. and Shimizu N. Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C. Biochem J. 270:583-9 (1990).

2. Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622 (1992)

3. Blackshear PJ. The MARCKS family of cellular protein kinase C substrates. J. Biol. Chem. 268: 1501-1504 (1993).

4. Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948 (1995)

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