Protocols:
Formulations for commonly used buffers and media
Immunostaining of cells in tissue culture
Immunostaining of tissue sections
N-terminal Peptide Sequencing from SDS-PAGE Electroblots
SDS-Polyacrylamide Gel Electrophoresis
Using Microsoft Excel (tm) to interpret and plot ELISA data
Useful On-Line computer Programs:
Translate a protein sequence into a DNA sequence for optimal expression in different species
Calculate solution volumes to make SDS-PAGE resolving and stacking gels
Calculate solution volumes to make SDS-PAGE resolving and stacking gels using Biorad 40% acrylamide
Calculate how much substance to weigh out to make a solution of a specific volume and molarity
Calculate the molecular weight of a mouse selected protein sequence
Calculate the absorbance coefficent of a protein at 280nm from the amino acid sequence
Calculate the molecular weight of a mouse selected nucleic acid sequence
Centrifugation: To find the RCF given by a particular rotor at a particular RPM
Centrifugation: To find the RPM to set a particular rotor at for a specific RCF
Calculate the blood volume of a rodent from the body weight