Calculate molecular weight and GC content from a DNA or RNA sequence:

This program will take a nucleic acid sequence and calculate the molecular weight and GC content. The program will ignore numbers, spaces or characters like B or Z which do not correspond to one of the 4 DNA bases (G, T, A and C) and the additional RNA base (U). It was actually quite interesting to research how to make this program. The atomic weights for each atom used are from the International Union of Pure and Applied Chemistry (IUPAC) web site here. This data gives average atomic weights based on average isotopic content on the planet earth, which can of course vary somewhat. The values, in Daltons, are H=1.00794, C=12.0107, N=14.0067, O=15.9994 and P=30.973761. These figures should give you far greater accuracy than you are likely to need, and the program actually rounds to the nearest hundredth of a Dalton. The program assumes that each phosphate group has one hydrogen atom bound to it, which may not be true outside the physiological pH range. This version can also deal with FASTA format sequences (see here for info on that); it ignores any line of text which is started by a ">" character, and keeps on ignoring until there is a newline character. The program can also process multiple FASTA format entries, only counting the sequence data and ignoring the stuff following the ">".

Please type or paste your DNA or RNA sequence in box below, can be upper or lowercase, program will read either and a mix of both.

Select button at left for RNA sequence, default is DNA sequence:

RNA sequence DNA sequence

Disclaimer: This program was constructed primarily to save time for busy researchers around the world. We hope you find it useful, and we are confident that it is accurate and reliable. However, we cannot be held responsible for any problems which may arise as a result of the use of this program.

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