SDSPolyacrylamide Gel Electrophoresis (SDSPAGE) Calculator For Use With BioRad 40% Acrylamide/Bisacrylamide Solution Below is a calculator for figuring out how much solution to make up for a particular total volume of gel, assuming you are using one of the popular ready made BioRad 40% acrylamide/bisacrylamide solutions (40% acrylamide 19:1 bisacrylamide catalogue #1610144 and #1610145, 40% acrylamide 29:1 bisacrylamide catalogue #1610146 and #1610147 and 40% acrylamide 37.5:1 bisacrylamide catalogue #1610148 and 1610149). BioRad also sells 40% acrylamide with no bisacrylamide, so you can make up any ratio of acrylamide to bisacrylamide that you want (catalogue #1610140 and #1610141). Just determine the volume of your SDSPAGE apparatus and use this to calculate how much of each solution to add. This can save you a lot of wasted solution! For a more extensive description of SDSPAGE and how it works go here.  


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