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SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Calculator:

    Below is a calculator for figuring out how much solution to make up for a particular total volume of gel. Just determine the volume of your apparatus and use this to calculate how much of each solution to add. This can save you a lot of wasted solution! For a more extensive description of SDS-PAGE and how it works go here.

Resolving Gel
Enter total volume of SDS-PAGE resolving gel solution required:
Enter percentage acrylamide concentration:

Stacking Gel
Enter total volume of SDS-PAGE stacking gel solution required:
Enter percentage acrylamide concentration (typically 4.44%):

Disclaimer: This program was constructed primarily to save time for busy researchers around the world. We hope you find it useful, and we are confident that it is accurate and reliable. However, we cannot be held responsible for any problems which may arise as a result of the use of this program.

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