Ubiquitin C-terminal Hydrolase 1 (UCHL1) is a small cytoplasmic enzyme of neurons and also one of the most abundant proteins of the nervous system. As a result UCHL1 may leak from damaged and degenerating neurons following trauma or degeneration. We discovered UCHL1 as a major stable protein both in extracts of bovine brain and it the cerebrospinal fluid (CSF) of aneurysmal subarachnoid hemorrhage (ASAH) patients (1). We developed our own antibodies and, using them, an ELISA to this protein and was able to show that it was released in large amounts into the CSF of ASAH patients in a manner which very closely matched that of pNF-H, which we had studied in the same patients previously (2).
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1. Lewis SB, Wolper R, Chi YY, Miralia L, Wang Y, Yang C and Shaw G. Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage. J Neurosci Res. 88:1475-84 (2010).
2. Lewis SB, Wolper RA, Miralia L, Yang C and Shaw G. Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. J Cereb Blood Flow Metab. 28:1261-71 (2008).