Supplemental Data on MCA-5C10, Monoclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
Our mouse monoclonal antibody to GFAP, MCA-5C10, is a robust marker of astrocytes in standard formalin fixed and paraffin embedded sections, as shown here on a section of adult horse brain.
Legend: This is one of a series of formalin fixed paraffin embedded horse brain samples generated by Maureen T. Long in the Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida. The brains from this series were removed whole, sliced incompletely cross ways and fixed over a 10-20 day period. The whole brain was put in a large necropsy specimen bucket containing 1-2 gallons of fixative, and after 24 hours, the formalin was poured off and then fresh formalin was added. The samples were fixed and embedded in paraffin in 2007 and sections were kept at room temperature until 2011. The sections were processed for antigen retrieval by boiling in pH=6 Citrate buffer for 10 min. Primary incubation with MCA-5C10 was for 1 hour at 37°C, and secondary antibody incubation and color reaction was performed using the Vector mouse ABC kit.
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