December 2023 News
We now have new data on two rabbit polyclonal antibodies to microtubule associated protein 2, RPCA-MAP2A/B and a new antibody RPCA-MAP2D. The MAP2 gene produces several different sized proteins by alternate transcription. MAP2C and MAP2D are lower molecular weight isotypes which are expressed early in development and are around 70kDa molecular size when run on […]
August 2023 News
We release yet more novel antibodies. We made a novel rabbit polyclonal and a mouse mpnoclonal to the enzyme catalase, RPCA-Catalase and MCA-6H14, both good markers odf peroxisomes. We also raised rabbit polyclonal antibodies against human Sigma-1 receptor RPCA-Sigma-1r and human γ-synuclein, RPCA-SNCG. We document that all work well on western blots for IF, ICC […]
June 2023 News
We have now finished testing all our rabbit polyclonal antibodies for immunohistochemistry on formalin fixed and paraffin embedded (FFPE) rodent and rodent tissues. So we found that many of them work very well, as shown in the examples below. So now we can recommend (or not) all of our antibodies on FFPE human and rodent […]
April 2023 News
Our recent work on the Uman NF-L antibodies published in pre-print form in BioRχiv was cited for the first time, in JAMA (the Journal of the American Medical Association), only the world’s highest impact medical journal! For a download see here. This work is now published in peer reviewed and freely downloadable form from the […]
March 2023 News
The data in our recent publication in BioRχiv preprint, entitled “Uman Type NF-L Antibodies Are Effective Reagents for the Imaging of Neurodegeneration” was edited and modified to include further data and was submitted for peer review at the journal “Brain Communications“. It has now been accepted for publication with the same title except that “NF-L” […]
December 2022 News
We release two new protein constructs, PROT-r-NF-L-Rct and PROT-r-NF-L-Stan. These constructs are designed to be used as protein standards for current neurofilament NF-L antibody based assays, such as the Uman/Quanterix NF-LIGHT™ and Quanterix Simoa™ assays. The two antibodies used in these assays both bind to the center of the “Coil II” region of α-helical “Rod” […]
September 2022 News
Our recent progress with the characterization of NF-L antibodies allow us to generate a novel and useful new reagent. This is our NF-L biomarker assay standard PROT-r-NF-L-Stan. This is a small recombinant protein which includes the epitopes for the two antibodies used in the NF-LIGHT™ and Quanterix Simoa™ NF-L biomarker assays. The recombinant construct is […]
August 2022 News
The Uman Diagnostics NF-LIGHT™ ELISA is a widely used ELISA type assay based on two mouse monoclonal antibodies to the neurofilament NF-L protein. One Uman antibody called variously UD1 or 2.1 is the detection reagent and the other, UD2, also known as 47.3, is the capture reagent (see Norgren et al. 2002). The same pair […]
Recombinant Human NF-L Rod 80-400
Human NF-L sequence was based on that was NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green). This construct is designed to express […]
Recombinant Full Length Human NF-L
Human NF-L sequence was based on that was NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green). MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFMSSFSYEP […]
Recombinant Human NF-L Coil II 256-400
Human NF-L sequence was based on that in NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green). This construct expresses the entire α-helical […]
October 2021 News
We release two high quality mouse monoclonal antibodies MCA-1B63 and MCA-3H25 to the S-tag, a 15 amino acid sequence originally identified as part of the N-terminal sequence of secreted bovine pancreatic ribonuclease by Frederick M. Richards in the 1950s. The S-tag sequence has been incorporated into many expression vectors and so S-tag antibodies are useful […]
July 2021 News
You may have noticed that we recently updated our homepage, as our previous version became unstable for some unknown probably computer code related reason. We hope you like the newer version. We are glad to report that our recent paper, “TNFα increases tyrosine hydroxylase expression in human monocytes”, an early version of which was posted […]
April 2021 News
We publish another collaboration between scientists at the University of Florida and EnCor, “TNF increases tyrosine hydroxylase expression in human monocytes” by Madison et al., BioRΧiv 10.1101/2021.04.13.439627v1, It describes in detail an ELISA developed using EnCor antibodies which can be used for quantitation of tyrosine hydroxylase (TH) levels in a variety of experimental and clinical contexts. […]
February 2021 News
We published a preprint on the BioRΧiv server, observations on the biomarkers pNF-H and UCHL1 in the bodily fluids of patients recovering from a common type of thoracic surgery. This report “A study of the neuronal injury biomarkers pNF-H and UCHL1 in serum, CSF and urine in a cohort of thoracic endovascular aortic repair (TEVAR) […]
January 2021 News
We release novel antibodies to one of the CasΦ, a.k.a. Cas-12j, family of enzymes. These are newly described and exceptionally compact enzymes of great potential utility in gene editing by CRISPR. Recent ecosystem and microbiome DNA sequencing and assembly characterized numerous whole genomes of “huge phages” or megaphages, see Al-Shayeb et al. Nature 578:425-431 (2020). […]
December 2020 News
We add new data concerning our three SARS-CoV2 spike protein cell binding domain antibodies which, we now show, strongly bind to Vero cells infected with patient derived SARS-CoV2 virus. Vero cells are a cell line derived from monkey kidney which express a membrane localized ACE2 receptor very similar to the human form and are therefore […]
August 2020 News
We add another mouse monoclonal antibody to the astrocytic and biomarker protein GFAP, MCA-3E10. This antibody was made against recombinant human GFAP and the epitope and kinetics of this antibody are known. It has utility as a component of assays designed to detect GFAP in blood, plasma and sera, which has become of potential interest […]
