{"product_id":"mouse-monoclonal-antibody-to-neurofilament-nf-l-nfl-nefl-cat-mca-da2rec","title":"Recombinant Mouse Monoclonal Antibody to Neurofilament NF-L (Nfl, NEFL), Cat# MRA-DA2","description":"\u003cp\u003e      The \u003ca href=\"https:\/\/encorbio.com\/products\/mouse-monoclonal-antibody-to-neurofilament-nf-l-cat-mca-da2\" target=\"_blank\" rel=\"noopener\"\u003eMCA-DA2\u003c\/a\u003e antibody was made against a preparation of neurofilament light chain NF-L, (a.k.a. NfL and NEFL) isolated from pig spinal cord. The antibody works well for western blotting and for IF, ICC and IHC on a variety of species including human, rat and mouse (for IHC see data under \"Additional Data\" tab). We epitope mapped this antibody to a short peptide in the C-terminal \"tail\" region of the molecule within the sequence SYYTSHVQEEQIEVE, amino acids 441-455 of the human sequence. We also found that the epitope for this antibody is rapidly degraded during neurodegeneration so this antibody works in a fashion complementary to our novel Degenotag™ reagents, see our recent paper for details (7). We cloned and sequenced this antibody and made a recomninant form. \n  \n  An alternate mouse monoclonal antibody made against recombinant full length human NF-L is \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/encorbio-com.myshopify.com\/products\/mouse-monoclonal-antibody-to-neurofilament-nf-l-cat-mca-1b11\" target=\"_blank\"\u003eMCA-1B11\u003c\/a\u003e, which recognizes an epitope in the α-helical coiled coil region of NF-L (7). Also available from EnCor are rabbit and chicken polyclonal antibodies to NF-L made against recombinant full length human NF-L, \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/encorbio-com.myshopify.com\/products\/rabbit-polyclonal-antibody-to-neurofilament-nf-l-cat-rpca-nf-l\" target=\"_blank\"\u003eRPCA-NF-L\u003c\/a\u003e, and \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/encorbio-com.myshopify.com\/products\/chicken-polyclonal-antibody-to-neurofilament-nf-l-cat-cpca-nf-l\" target=\"_blank\"\u003eCPCA-NF-L\u003c\/a\u003e. All four antibodies work on a variety of species and are clean and specific on western blots, cell and tissue staining. We also generated a polyclonal antibody \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/encorbio-com.myshopify.com\/products\/rabbit-polyclonal-antibody-to-nf-l-c-terminus-cat-rpca-nf-l-ct\" target=\"_blank\"\u003eRPCA-NF-L-Ct\u003c\/a\u003e to the C-terminus of NF-L, which like MCA-DA2, binds an epitope destroyed on neurodegeneration.\u003c\/p\u003e","brand":"EnCor Biotechnology","offers":[{"title":"100µL of 1mg\/mL","offer_id":49157535072508,"sku":null,"price":250.0,"currency_code":"USD","in_stock":true},{"title":"500µL of 1mg\/mL","offer_id":49157535105276,"sku":null,"price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0676\/5307\/5196\/files\/MRA-DA2-NF-L_RPCA-GFAP_rat_CB_10X_IFC.jpg?v=1787937814","url":"https:\/\/encorbio.com\/products\/mouse-monoclonal-antibody-to-neurofilament-nf-l-nfl-nefl-cat-mca-da2rec","provider":"EnCor Biotechnology","version":"1.0","type":"link"}