HeLa cells stained with mouse monoclonal antibody to c-Fos, showing strong induction of c-Fos expression following stimulation with serum.
HeLa cells were serum-starved (Left) or serum-starved and then stimulated with 20% fetal bovine serum (FBS) for 2 hours (Right). The were then stained under identical conditions with our monoclonal antibody to c-Fos, MCA-2H2 (green) and our chicken anti-vimentin (CPCA-Vim, red). Nuclear DNA was revealed with DAPI (blue). Serum starvation inhibits c-Fos expression, while treatment with FBS for 2 hours strongly stimulates c-Fos expression which localizes in the nucleus as shown. The c-Fos antibody was used at a dilution of 1:1,000 from a 1mg/mL solution. The vimentin antibody was used at a dilution of 1:100,000. Cultures were processed using our standard fixation and staining procedure (described here). To order the c-Fos antibody go to our order form (here) or use our online store here. Picture taken with a Zeiss 40X objective and documented with a SPOT camera. Mouse click on each image to get an enlarged view.