GFAP, chicken polyclonal, Cat# CPCA-GFAP

GFAP, chicken polyclonal, Cat# CPCA-GFAP



Mixed cultures of neurons and glia stained with CPCA-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Western blot of whole rat brain (lane1) and mouse brain (lane2) homogenate  stained with CPCA-GFAP, at dilution of 1:5,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to GFAP. Also shown is protein size marker in red. Note, molecular weight of mouse GFAP is smaller than that of rat GFAP.

Product name Anti-GFAP
Description Chicken Polyclonal to Anti-Glial Fibrillary Acidic Protein, GFAP
Reference Code CPCA-GFAP
HGNC name GFAP
RRID# AB_2109953
Molecular weight ~55 kDa
Immunogen Recombinant construct of human GFAP protein
Isotype IgY
Concentration Antibody is supplied as an aliquot of concentrated IgY preparation
Species Reactivity Human, chicken, rat, mouse
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blot: 1:10,000. IF/ICC and IHC: 1:1,000-1:5,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and coworkers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kDa protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.

In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander’s disease was recently shown to be caused by point mutations in the protein coding region of the GFAP gene (2). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.

The antibody was made against a recombinant construct of human GFAP protein. This antibody has been used in several recent publications (see 4). The HGNC name for this protein is GFAP.


References:

1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972.

2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 2001

3. Liem RKH, Yen SH, Salomon GD and Shelanski ML. Intermediate filaments in nervous tissues. J Cell Biol 79:637-745 (1978).

4. Bruijnzeel AW, Bauzo RM, Munikoti V, Rodrick GB, Yamada H, Fornal CA, Ormerod BK, Jacobs BL.Tobacco smoke diminishes neurogenesis and promotes gliogenesis in the dentate gyrus of adolescent rats. Brain Res. 1413:32-42 (2011).

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