View of mixed neuron/glial cultures stained with CPCA-MAP2 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.
|Western blot of extract of adult rat brain crude lysate (lane 1) E20 rat brain extract (lane 2) and adult mouse brain extract (lane 3) stained with CPCA-MAP2, revealing a band at ~280 kDa. Antibody was used at a concentration of 1:50,000. Mouse MAP2 is known to run a little lower on SDS-PAGE than the rat protein. The S lane contains molecular weight standards of the indicated molecular size.
||Chicken Polyclonal to Anti-Microtubule Associated Protein 2, MAP2
||Recombinant human projection domain sequence, amino acids 377-1505, our products Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
||Antibody is supplied as an aliquot of IgY preparation.
||Human, horse, cow, pig, chicken, rat, mouse
||Western blot, IF/ICC, IHC
|Suggestions for use
||Western blot: 1:20,000. IF/ICC and IHC: 1:10,000-1:20,000.
||Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one α-subunit and one β-tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t
(tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels.
All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau).
MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. The immunogen for this antibody is comprised of three protein constructs spanning the projection domain of MAP2 (amino acids 377-1505 of the human sequence), specifically our products Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3. The HGNC name for this protein is MAP2.
1. Goetz AK, Scheffler B, Chen HX, Wang S, Suslov O, Xiang H, Brüstle O, Roper SN, Steindler DA. Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells. Proc Natl Acad Sci U S A. 103:11063-11068 (2006).
2. Walton NM, Snyder GE, Park D, Kobeissy F, Scheffler B, Steindler DA. Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation. Stem Cells 27:280-289 (2009).
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