Microtubule Associated Protein 2, MAP2, mouse monoclonal, Cat# MCA-5H11

Microtubule Associated Protein 2, MAP2, mouse monoclonal, Cat# MCA-5H11



Mixed neuron/glia cultures stained with MCA-5H11 (green) and also rabbit antibody of neurofilament NF-H RPCA-NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).

Western blot of whole rat brain lysate probed with MCA-5H11 antibody to MAP2. Note that the strong single band running at about 280Kda corresponds to MAP2.

Product name Anti-MAP2
Description Mouse Monoclonal to Anti-Microtubule Associated Protein 2, MAP2
Reference Code MCA-5H11
HGNC name MAP2
RRID# AB_2572347
Molecular weight ~250 kDa by SDS-PAGE
Immunogen Full length purified bovine protein. Epitope maps to 1057-1507 of the human sequence.
Isotype IgG2b
Concentration Antibody is supplied as an aliquot of 1 mg/mL of affinity purified antibody.
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blot: 1:10,000. ICC/IF and IHC: 1:1,000.
Storage instructions Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (a.k.a. MAP-tau or tau).

MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers of neuronal cells, their perikarya and neuronal dendrites. In contrast MAP-t (MAP-tau) is found predominantly but not exclusively in neuronal axons. The HGNC name for this protein is MAP2.


References:

1. Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 6:204 (2004)

2. Vallee R. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J. Cell Biol. 92:435-442 (1992)

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