EnCor Biotechnology
Rabbit Polyclonal Antibody to β-synuclein RPCA-SNCB
Description
The RPCA-SNCB antibody was made against full length human recombinant β-synuclein protein expressed in and purified from E.Coli. This antibody recognizes full length human and rodent β-synuclein specifically both in western blots and in immunocytochemical experiments. EnCor also supplies a high quality monoclonal antibody to β-synuclein MCA-6A10, and high quality antibodies to α-synuclein MCA-2A7 and CPCA-SCNA.
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| Name: | Beta-synuclein, rabbit polyclonal antibody, Cat# RPCA-SNCB |
| Immunogen: | Full length human recombinant β-Synuclein protein expressed in and purified from E. Coli |
| HGNC Name: | SNCB |
| UniProt: | Q16143 |
| Molecular Weight: | ~15kDa |
| Host: | Rabbit |
| Species Cross-Reactivity: | Human, Rat, Mouse, Cow, Pig |
| RRID: | AB_2860578 |
| Format: | Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC |
| Recommended Dilutions: | WB: 1:1,000-2,000. IF/ICC 1:1,000-2,000. IHC 1:2,000. |
| Storage: | Store at 4°C for short term, for longer term store at -20°C |
β-synuclein is a member of the synuclein protein family, the other two members being α and γ-synuclein, each protein being coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo (1), and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, so that it is a major component of the Lewy bodies of Parkinson’s disease (2-5). β-synuclein was isolated as a component of normal and diseased human brain as a protein clearly related to but distinct from α-synuclein (6). The human β-synuclein molecule is 134 amino acids in size compared to 140 amino acids for α-synuclein, and the N-terminal halves of the two molecules are virtually identical while the C-terminal regions is more variable. As a result we made our new β-synuclein antibodies to this region. Like α-synuclein, β-synuclein is heavily concentrated in the brain in presynaptic regions. A third synuclein, γ-synuclein was originally identified as breast cancer specific gene 1, (BCSG1), but is also heavily expressed in brain and also has a similar N-terminal sequence (7). The three synucleins appear to have overlapping functions so genetic deletion of all three in mice is required to obtain serious neurological deficits (7).
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded mouse hippocampus section with rabbit pAb to β-synuclein, RPCA-SNCB, dilution 1:2,000, detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. β-synuclein is abundant in pre-synaptic regions of the brain. This antibody performs well in testing with both 4% PFA and standard NBF fixed mouse, human, and rat tissues. Mouse select image for larger view.
1. Maroteaux L, Campanelli JT, Scheller RH. Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal. J. Neurosci. 8:2804-15 (1988).
2. Lavedan C. The Synuclein Family. Genome Research 8:871-80 (1998).
3. Polymeropoulos, MH et al. Mutation in the alpha-synuclein gene identified in families with Parkinson’s disease. Science 276:2045-7 (1997).
4. Kruger, R et al. Ala30-to-Pro mutation in the gene encoding alpha-synuclein in Parkinson’s disease. Nature Genet. 18:106-8 (1998).
5. Chartier-Harlin, M-C. et al. Alpha-synuclein locus duplication as a cause of familial Parkinson’s disease. Lancet 364:1167-9 (2004).
6. Ji H. et al. Identification of a breast cancer-specific gene, BCSG1, by direct differential cDNA sequencing. Cancer Res. 57:759-64 (1997).
7. Greten-Harrison, B. et al. αβγ-Synuclein triple knockout mice reveal age-dependent neuronal dysfunction. PNAS 107:19573-8 (2001).
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