Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-L, CPCA-NF-L, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, <a href="http://encorbio.com/products/mca-1b7/">MCA-1B7</a>, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. CPCA-NF-L antibody labels perikarya and processes of neuronal cells,
Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-L, CPCA-NF-L, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, <a href="http://encorbio.com/products/mca-1b7/">MCA-1B7</a>, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. CPCA-NF-L antibody labels perikarya and processes of neuronal cells,

EnCor Biotechnology

Chicken Polyclonal Antibody to Neurofilament NF-L (Nfl, NEFL) Cat# CPCA-NF-L

$250.00
Description

      The CPCA-NF-L antibody was made against a preparation of full length human recombinant NF-L protein. It binds NF-L from a variety of species including human, rat and mouse. We document that the antibody works well not only for western blotting, IF and ICC but also on formalin fixed paraffin embedded sections, select the "Additional Info" for this data. We also generated highly specific rabbit polyclonal antibodies, RPCA-NF-L and RPCA-NF-L-ct, and several mouse monoclonal antibodies which have been epitope mapped and bind different forms of NF-L, MCA-DA2, MCA-6H63, and MCA-1D44.
Amount: 100µL of IgY prep
Amount: 100µL of IgY prep
Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-L, CPCA-NF-L, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN, MCA-1B7, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. CPCA-NF-L labels perikarya and processes of neuronal cells, especially the axons of basket cells, while the FOX3/NeuN antibody stains the nuclei and proximal cytoplasm of neurons.
Western blot analysis of tissue lysates probed with chicken pAb to NF-L, CPCA-NF-L, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord and [6] cow spinal cord. Strong bands at ~68kDa corresponds to NF-L proteins which are known to have slightly different apparent SDS-PAGE molecular weights across species boundaries.

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Name: NF-L, chicken polyclonal antibody, Cat# CPCA-NF-L
Immunogen: Recombinant human NF-L protein
HGNC Name: NEFL
UniProt: P07196
Molecular Weight: 68-70kDa by SDS-PAGE
Host: Chicken
Species Cross-Reactivity: Human, rat, mouse, cow, pig
RRID: AB_2149931
Format: Concentrated IgY preparation plus 0.02% NaN3
Applications: WB, IF/ICC, IHC
Recommended Dilutions: WB: 1:20,000. IF/ICC: 1:2,000. IHC: 1:4,000
Storage: Store at 4°C.

      Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like CPCA-NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others (2-4). Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration (5).



Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum with chicken pAb to NF-L, CPCA-NF-L, dilution 1:4,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The NF-L antibody detects perikaryal and dendritic processes of Purkinje cells and axons of other neuronal cells, particularly basket cells. Mouse select image for larger view.

1. Hoffman et al. Neurofilament gene expression:a major determinant of axonal caliber. PNAS 84:3472-6 (1987).
2. Perrot R, et al. Review of the Multiple Aspects of Neurofilament Functions, and their Possible Contribution to Neurodegeneration. Mol. Neurobiol. 38:27-65 (2008).
3. Lépinoux-Chambaud C. Eyer J. Review on intermediate filaments of the nervous system and their pathological alterations. Histochem. Cell Biol. 140:13-22 (2013).
4. Liu Q. et al. Neurofilamentopathy in Neurodegenerative Diseases. Open Neurol. J. 5:58–62 (2011).
5. Bacioglu M, et al. Neurofilament light chain in blood and CSF as marker of disease progression in mouse models and in neurodegenerative diseases. Neuron 91:56-66 (2016).

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