EnCor Biotechnology

Recombinant Full Length Human MAP2D Protein Cat# Prot-r-MAP2D

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Description

      Microtubule associated protein 2 (MAP2) is a major protein of neurons concentrated in dendrites and perikarya but absent from axons. A single mammalian MAP2 gene generates multiple lower molecular weight forms usually named MAP2A, MAP2B, MAP2C and MAP2D. MAP2D is the second smallest variant and is expressed early in neuronal development. We have expressed the full length human MAP2D sequence based on the human sequence reported in NP_001034627.1.

Amount: 50µg
Amount: 50µg
      The S lane shows molecular weight standards of the indicated apparent molecular weights in kiloDaltons. The next 5 lanes show 2µg amounts of purified recombinant forms of various human MAP2 proteins. D is recombinant full length MAP2D and C is recombinant full length MAP2C. MAP2D is 559 amino acids while MAP2C is 471, missing one of the microtubule binding domains of MAP2D and a more N-terminal 57 amino acid N-terminal sequence. Lanes P1, P2 and P3 show each recombinant segments of the human MAP2 projection domain, found in MAP2A and B but absent from MAP2C and D. The P1 construct is amino acids 233-684, the P2 is 712-1136 and P3 is 1137-1588. All of the constructs run more slowly on SDS-PAGE than expected from their molecular size, probably due to their high content of charged amino acids. These constructs were used to make a series of mouse monoclonal antibodies. The lane marked BSA contains 2µg of bovine serum albumin, another gel standard.

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Name: MAP2C, recombinant construct, PROT-r-MAP2D
HGNC Name: MAP2D
UniProt: P11137
Molecular Weight: 64.7kDa, ~75kDa by SDS-PAGE
Format: 1mg/mL in 6M Urea
Applications: Protein standard, immunogen, ELISA

      The sequence is from REFSEQ XP_006712595. This is one of the longer but still low molecular weight transcripts of the human MAP2 gene, lacking projection domain sequence but including all 4 of the C-terminal microtubule binding domains. The construct was expressed in pET29a and has a few N and C-terminal amino acids derived from the vector, including a C-terminal His-tag.

      This is the second shortest transcript of the human MAP2 gene, including 4 of the C-terminal microtubule binding domains. The sequence is identical to that in NP_001034627.1. The construct was expressed in pET29a and has a few N and C-terminal amino acids derived from the vector, including a C-terminal His-tag. This sequence includes an S-tag affinity peptide (red), an enterokinase cleavage site (green) and the His tag (underlined).


MKETAAAKFE RQHMDSPDLG TLVPRGSMAD IGSEFMADER KDEAKAPHWT SAPLTEASAH
SHPPEIKDQG GAGEGLVRSA NGFPYREDEE GAFGEHGSQG TYSNTKENGI NGELTSADRE
TAEEVSARIV QVVTAEAVAV LKGEQEKEAQ HKDQTAALPL AAEETANLPP SPPPSPASEQ
TVTVEEAAGG ESALAPSVFK QAKDKVSNST LSKIPALQGS TKSPRYSSAC PSTTKRATFS
DSLLIQPTSA GSTDRLPYSK SGNKDGVTKS PEKRSSLPRP SSILPPRRGV SGDRDENSFS
LNSSISSSAR RTTRSEPIRR AGKSGTSTPT TPGSTAITPG TPPSYSSRTP GTPGTPSYPR
TPHTPGTPKS AILVPSEKKV AIIRTPPKSP ATPKQLRLIN QPLPDLKNVK SKIGSTDNIK
YQPKGGQVRI LNKKIDFSKV QSRCGSKDNI KHSAGGGNVQ IVTKKIDLSH VTSKCGSLKN
IRHRPGGGRV KIESVKLDFK EKAQAKVGSL DNAHHVPGGG NVKIDSQKLN FREHAKARVD
HGAEIITQSP GRSSVASPRR LSNVSSSGSI NLLESPQLAT LAEDVTAALA KQGLVDVDKL AAALEHHHHH H

Number of amino acids: 611
Molecular weight: 64708.2
Theoretical pI: 9.55

Amino acid composition:
Ala (A) 57 9.3%
Arg (R) 34 5.6%
Asn (N) 21 3.4%
Asp (D) 27 4.4%
Cys (C) 3 0.5%
Gln (Q) 22 3.6%
Glu (E) 38 6.2%
Gly (G) 49 8.0%
His (H) 20 3.3%
Ile (I) 27 4.4%
Leu (L) 38 6.2%
Lys (K) 49 8.0%
Met (M) 4 0.7%
Phe (F) 10 1.6%
Pro (P) 51 8.3%
Ser (S) 76 12.4%
Thr (T) 44 7.2%
Trp (W) 1 0.2%
Tyr (Y) 7 1.1%
Val (V) 33 5.4%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 83

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.248, assuming all pairs of Cys residues form cystines

Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.246, assuming all Cys residues are reduced

1. Dehmelt H, Halpain S. The MAP2/Tau family of microtubule-associated proteins.
Genome Biol. 204 (2005)

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