SDS-PAGE gel of recombinant human ACE2 virus binding region. Lane 1 shows protein molecular weight standards of apparent size indicated in kiloDaltons. Lanes 2 and 3 are 5.6μg and 2.8μg BSA and 4 and 5 are the ACE2 construct running at an apparent molecular weight about 70kDa, somewhat higher than the 63kDa predicted from the amino acid sequence. This discrepancy is likely due to the highly acidic nature of the protein. The protein was expressed in E. coli and extracted from inclusion bodies using 6M urea.
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| Name: | ACE2 SARS-CoV1 and SARS-CoV2 binding domain |
| HGNC Name: | ACE2 |
| UniProt: | Q9BYF1 |
| Molecular Weight: | 63kDa, 70kDa by SDS-PAGE |
| RRID: | Pending |
| Format: | Supplied as 1mg/mL in 6M urea, 10mM phosphate buffer pH=7.5 |
| Applications: | Antibody generation, ELISA |
| Storage: | Stable at 4°C for several months. For longer term store at -20°C, minimize freeze/thaw cycles |
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3. Walls, A C et al. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell doi: 10.1016/j.cell.2020.02.058 180:1-12 (2020)
4. Yan, R et al. Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Science doi:10.1126/science.abb2762 367:1444–8 (2020).
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