Rabbit Polyclonal Antibody to MAP2AB RPCA-MAP2AB

      Microtubules are 25nm diameter protein rods found in eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 was originally named as one of the higher molecular weight MAPs with an SDS-PAGE molecular weight of about 280kDa (1-3). There is a single mammalian MAP2 gene which may generates two high molecular weight proteins of ~280kDa named MAP2A and MAP2B and multiple lower molecular weight forms usually named MAP2C and MAP2D which run on SDS-PAGE gels at 60-70kDa. The lower molecular weight forms are found in neurons early in development, but later are replaced by the higher molecular weight forms (2). The MAP2A and MAP2B forms include a protein insert missing in MAP2C and MAP2D which forms fine filamentous protrusions from the sides of brain microtubules referred to as the "projection domain". This antibody was made against the projection domain sequences and so is specific for MAP2A and MAP2B. MAP2 isoforms are expressed only in neuronal perikarya and dendrites so appropriate antibodies are used to identify these structures in cell culture and sections (for example images in references 4-8 used EnCor MAP2 antibodies).

Chromogenic Immunostaining of a NBF fixed paraffin embedded human brain cortex section with rabbit pAb to MAP2A/B, RPCA-MAP2AB, dilution 1:5,000. Staining was performed using detected with DAB (brown) with the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The RPCA- MAP2AB antibody specifically labels the cytoplasm (soma and dendrites) of neurons. This antibody performs well in testing with 4% PFA and standard NBF fixed mouse, rat and human tissue. Mouse select image for larger view.

1. Dehmelt H, Halpain S. The MAP2/Tau family of microtubule-associated proteins.
Genome Biol. 6:204 (2005).
2. Nunez J. Immature and mature variants of MAP2 and tau proteins and neuronal plasticity. Trends Neurosci. 11:477-9 (1998).
3. Vallee R. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J. Cell Biol. 92:435-42 (1992).
4. Goetz AK, et al. Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells. PNAS 103:11063-8 (2006).
5. Walton NM et al. Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation. Stem Cells 27:280-9 (2009).
6. Gasser A, et al. An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier. J. Neurosci. 32:7232-43 (2012).
7. Rush AM, et al. Differential modulation of sodium channel Nav1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily. Eur. J. Neurosci. 23:2551-62 (2006).
8. Eckenstein FP, McGovern T, Kern D, Deignan J. Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor. Exp. Neurol. 198:338-49 (2006).