| Name: | Chicken polyclonal antibody to MAP-Tau |
| Immunogen: | Recombinant full length version of the shortest human Tau isoform purified from E. coli. |
| HGNC Name: | MAPT |
| UniProt: | P10636 |
| Molecular Weight: | 48-67kDa by SDS-PAGE |
| Host: | Chicken |
| Isotype: | |
| Species Cross-Reactivity: | Human, rat, mouse, cow, pig, horse |
| RRID: | AB_2572348 |
| Format: | Concentrated IgY preparation in PBS plus 0.02% NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:10,000. IF/ICC 1:2,000 and IHC: 1:10,000. |
| Storage: | Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage |
Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-τ, CPCA-Tau, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, MCA-4H5, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The CPCA-Tau antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.
Western blot analysis of tissue lysates using chicken pAb to MAP-τ, CPCA-Tau, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.
Chicken Polyclonal Antibody to Tau, MAP-τ
Cat# CPCA-Tau
$120.00 – $800.00
Tau is a low molecular weight member of the microtubule associated protein or MAP family. Several serious human diseases are associated with accumulations of tau protein, most notably the neurofibrillary tangles of Alzheimer’s disease. Accumulations of tau in neurons are also characteristic of chronic traumatic encephalopathy, Pick’s disease and several other neurodegenerative diseases. Together these disorders are known as “tauopathies”. The single mammalian tau gene produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms ranging from 48-67kDa by SDS-PAGE predominate, though larger isotypes are seen mostly in the peripheral nervous system. The tau molecules are very heavily charged and run on SDS-PAGE much more slowly than predicted from their real molecular size. For example the smallest human tau isotype runs at 48kDA on SDS-PAGE but the real molecular weight is 32kDa. Tau proteins are substrates for ser/thr phosphorylation and other post-translational modifications.
The CPCA-Tau antibody was raised against a recombinant form of one of the lower molecular weight human tau isoform, specifically the human 441 amino acid htau40 form described by Goedert et al. We have two mouse monoclonal antibody raised against the same form of human tau, MCA-2E9 and MCA-5B10. Both antibodies recognize the unphosphorylated forms of MAP-τ and have been epitope mapped to distinct regions of the molecule. CPCA-Tau works well on western blots and IF and formalin fixed paraffin sections. Mouse select image at left for larger view.
Chromogenic Immunostaining of a formalin fixed paraffin embedded human cortical section
Tissue from an Alzheimer’s disease patient was stained with CPCA-Tau, dilution 1:10,000, and detected using DAB (brown) using the ABC method. Hematoxylin (blue) was used as the counterstain. The CPCA-Tau antibody labels neurofibrillary tangles in the cytoplasm of diseased neurons producing a characteristic flame shaped morphology and also highlights dystrophic neurites associated with senile plaques. Mouse select image for larger view.
1. Weingarten, M. D., Lockwood, A. H., Hwo, S.-Y. and Kirschner, M. W. A protein factor essential for microtubule assembly. PNAS 72:1858–62 (1975).
2. Cleveland, D. W., Hwo, S. Y., Kirschner, M. W. Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin. J Mol. Biol. 116:207-25 (1977).
3. Goedert, M., Spillantini, M. G. A century of Alzheimer’s disease. Science 314:777–81 (2006).
4. Ballatore, C., Lee, V. M., Trojanowski, J. Q. Tau-mediated neurodegeneration in Alzheimer’s disease and related disorders. Nat. Rev. Neurosci. 8:663–72 (2007).
5. Wolfe, M. S. Tau mutations in neurodegenerative diseases. J. Biol. Chem. 284:6021-6025 (2009).
6. Goedert M, Spillantini MG, Crowther RA. Cloning of a big tau microtubule-associated protein characteristic of the peripheral nervous system. PNAS 89:1983-7 (1992).
7. Boyne LJ, Tessler A, Murray M, Fischer I. Distribution of Big tau in the central nervous system of the adult and developing rat. J. Comp. Neurol. 358:279-93 (1995).
