Chicken Polyclonal Antibody to VLP1
Cat# CPCA-VLP1

Visinin was originally isolated biochemically from chicken retina as a major protein of ~24kDa on SDS-PAGE gels (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2,3). One of these, visinin-like protein 1 is a low molecular weight protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4,5). The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four “EF hand” domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. The protein is 191 amino acids in size and has a molecular weight on SDS-PAGE of 18kDa. The protein has recently been suggested to be a useful blood biomarker of Alzheimer’s disease and traumatic brain injury (6-8).
The CPCA-VLP1 antibody was made against full length recombinant human VSNL1. It can be used to track VSNL1 by ELISA, on western blots and in cells in culture and sections, including formalin fixed and paraffin embedded human and rodent sections. We also manufacture mouse monoclonal antibodies and a rabbit polyclonal antibody to this protein, MCA-3A9, MCA-2D11 and RPCA-VLP1 respectively. Mouse select image at left for larger view.