| Product name | ELISA UCHL1 |
|---|---|
| Description | ELISA kit for the detection of UCHL1 in plasma, serum, CSF and tissue extracts. |
| Reference Code | ELISA UCHL1 |
| HGNC name | UCHL1 |
| Molecular weight | ~25kDa by SDS-PAGE |
| Immunogen | Recombinant full length human UCHL1 |
| Isotype | Mouse monoclonal capture and rabbit IgG detect |
| Species Reactivity | Human, horse, cow, pig, rat, mouse |
| Applications | ELISA of blood, CSF, urine and tissue extracts. |
| Storage instructions | Shipped on ice. Store at 4°C. |
UCHL1 ELISA
Cat# ELISA-UCHL1
$400.00 – $1,800.00
Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). The protein is extremely abundant and claimed to represent 1-2% of total brain protein (2). It was later found that an ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to UCHL1. UCHL1 is one of the numerous deubiquiting enzymes and is expressed in the CNS only in neuronal cells. This protein leaks into blood and CSF following various kinds of neuronal damage. As a result detection of this protein in blood or CSF, which can be performed with the EnCor assay, is a useful surrogate marker of ongoing neuronal damage.
See here for the manual for this kit.
Ubiquitin C-terminal hydrolase 1 (UCHL1) has several other names, such as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). The protein is extremely abundant, and was estimated to be present at a concentration of 200-500 micrograms/g wet weight, representing a major protein component of neuronal cytoplasm (1). This has been claimed to represent 1-2% of total brain protein (2). It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to UCHL1. This is the first of a family of ubiquitin C-terminal hydrolases which have been characterized, many of which also have rigid cell type specific expression patterns.
The kit contains one standard ELISA 96 well plate coated with the mouse monoclonal antibody which has been blocked and so is ready to use. The plate can be separated into 8 12 well strips. The kit includes enough of the rabbit anti UCHL1 detection antibody and a pure recombinant full length human UCHL1 protein standard. Also included are a goat anti-rabbit alkaline phosphatase conjugate, p-Nitrophenol phosphatase substrate and buffer concentrates. We also include detailed instructions.
| Name: | X-ELISA kit for detection of UCHL1 |
| Immunogen: | |
| HGNC Name: | UCHL1 |
| UniProt: | |
| Molecular Weight: | |
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| Isotype: | |
| Species Cross-Reactivity: | |
| RRID: | Pending |
| Format: | |
| Applications: | CSF and blood analysis |
| Recommended Dilutions: | |
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Peer reviewed publications using the EnCor assay;
1. Blyth BJ, et al. Elevated serum ubiquitin carboxy-terminal hydrolase L1 is associated with abnormal blood-brain barrier function after traumatic brain injury. J. Neurotrauma 28:2453-62 (2011).
2. Ringger NC, et al. Biomarkers of brain injury in foals with hypoxic-ischemic encephalopathy. J. Vet. Intern. Med. 25:132-7 (2011).
3. Douglas-Escobar M, et al. A pilot study of novel biomarkers in neonates with hypoxic-ischemic encephalopathy. Pediatr. Res. 68:531-6 (2010).
4. Lewis SB, et al. Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage. J. Neurosci. Res. 88:1475-84 (2010).
5. Morel AA, et al. Measurement of novel biomarkers of neuronal injury and cerebral oxygenation after routine vaginal delivery versus cesarean section in term infants. J. Perinat. Med. 42:705-9 (2014).
6. Puvenna V, et al. Significance of ubiquitin carboxy-terminal hydrolase L1 elevations in athletes after sub-concussive head hits. PLoS One 9:e96296 (2014).
1. Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J. Neurochem. 40:1542-7 (1983).
2. Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989).
3. Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson’s disease susceptibility. Cell 111:209-18 (2002).
4. Day IN, Thompson RJ. UCHL1 (PGP 9.5): Neuronal biomarker and ubiquitin system protein. Prog. Neurobiol. 90:327-62 (2009).
