Our fp506 construct is identical to that in the Uniprot database entry Q9U6Y5, as described in reference 1. The vector adds a C-terminal His-tag which was use to purify the protein and this, along with some other vector derived sequence, adds about 5 kDa to the molecule.
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science 263:802-5 (1994).
2. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML and Lukyanov SA. Fluorescent proteins from nonbiolu.minescent Anthozoa species Nat. Biotechnol. 17: 969-973 (1999).
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science 263:802-5 (1994).
2. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML and Lukyanov SA. Fluorescent proteins from nonbiolu.minescent Anthozoa species Nat. Biotechnol. 17: 969-973 (1999).
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