| Name: | Mouse Monoclonal to Lys20p and Lys21p |
| Immunogen: | Yeast nuclear preparation |
| HGNC Name: | NA |
| UniProt: | G2WBY3, G2WC30 |
| Molecular Weight: | 47.1 and 48.6kDa |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Saccharomyces cerevisiae |
| RRID: | AB_2572337 |
| Format: | Concentrated hybridoma tissue culture media plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:10,000-1:50,000. ICC/IF and IHC: 1:1,000-1:10,000. |
| Storage: | Store at 4°C for short term, for longer term at -20°C. Avoid freeze/thaw cycles. |
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for MCA-31F5 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.
Mouse Monoclonal Antibody to Lys20p/Lys21p
Cat# MCA-31F5
$120.00 – $800.00
Lys20p and Lys21p are two isozymes of the Baker’s yeast Saccharomyces cerevisiae homocitrate synthase. Homocitrate synthases catalyse the reaction of acetyl-coenzyme A, 2-oxoglutarate and water to produce 2-hydroxybutane-1,2,4-tricarboxylate + coenzyme A. Their systematic names of the isozymes at the yeast genome organization are YDL182W and YDL131Wrespectively. The Lys20p protein is of molecular size 47.1kDa and the Lys21p protein is 48.6kDa. They are produced by two distinct genes and the two molecules are ~90% identical at the amino acid level so it is not surprising that an antibody would recognize both molecules. The MCA-31F5 antibody was made by injecting a yeast nucleolus enriched fraction into mice to produce hybridoma lines (1). MCA-31F5 was selected as one of many hybridomas which bound yeast nuclei by immunofluoresecence. MCA-31F5 detected a closely spaced doublet on immunoblots of yeast extracts, and screening of a yeast λgt11 expression library identified a reactive clone which encoded Lys20p. Subsequent studies showed tat the MCA-31F5 showed equal reactivity with the closely related Lys21p. Both proteins are localized to the nucleoplasm of the nucleus.
Lys20p and Lys21p are two isozymes of the Baker’s yeast Saccharomyces cerevisiae homocitrate synthase. Homocitrate synthases catalyse the reaction of acetyl-coenzyme A, 2-oxoglutarate and water to produce 2-hydroxybutane-1,2,4-tricarboxylate + coenzyme A. Their systematic names of the isozymes at the yeast genome organization are YDL182W and YDL131Wrespectively. The Lys20p protein is of molecular size 47.1kDa and the Lys21p protein is 48.6kDa. They are produced by two distinct genes and the two molecules are ~90% identical at the amino acid level so it is not surprising that an antibody would recognize both molecules.
The MCA-31F5 antibody was made by injecting a yeast nucleolus enriched fraction into mice to produce hybridoma lines (1). MCA-31F5 was selected as one of many hybridomas which bound yeast nuclei by immunofluoresecence. MCA-31F5 detected a closely spaced doublet on immunoblots of yeast extracts, and screening of a yeast λgt11 expression library identified a reactive clone which encoded Lys20p. Subsequent studies showed tat the MCA-31F5 showed equal reactivity with the closely related Lys21p. Both proteins are localized to the nucleoplasm of the nucleus.
1. Chen S, Dove JE, Brockenbrough JS, and Aris JP. Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 272:10839-46.
