Name: | Mouse monoclonal antibody to PEA-15 |
Immunogen: | Full length human PEA-15 as expressed in and purified from E. coli. |
HGNC Name: | PEA-15 |
UniProt: | Q15121 |
Molecular Weight: | 15kDa |
Host: | Mouse |
Isotype: | IgG1 |
Species Cross-Reactivity: | Human, Rat, Mouse, Cow |
RRID: | AB_2861182. |
Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
Applications: | WB, ICC/IF |
Recommended Dilutions: | WB: 1:1,000-1:2,000. ICC/IF: 1:1,000-2,000. IHC not recommended |
Storage: | Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Mouse Monoclonal Antibody to PEA-15
Cat# MCA-4D2
$120.00 – $800.00
Pea15 was originally isolated as a major low molecular weight of embryonic mouse striatal astrocytes grown in cell culture. Three spots on 2D gels with an apparent molecular weight of 15kDa and isoelectric point 5.1-5.3 were shown to be different forms of one protein. The protein was serine phosphorylated on one site by protein kinase C both in vivo and in vitro and the protein was named “phosphoprotein enriched in astrocytes of 15kDa”, hence PEA-15 (1). Subsequent cloning and sequencing revealed a protein well conserved in sequence between mouse and human and which was heavily expressed in brain (2). Independently the same protein was found to be upregulated in fibroblasts and tissues of diabetic patients, and has hence named “protein enriched in diabetes” or PED (3). Immunocytochemical studies showed that the protein was heavily expressed in astrocytes and certain neurons in the CNS of mice, though it is expressed a lower levels ubiquitously (2,4). The protein could be phosphorylated on a second site by either CaMKII or Akt/PKB, and further examination of the amino acid sequence revealed an N-terminal death effector domain (DED), a predominantly α-helical domain found in many proteins which function in the regulation of apoptosis (5). PEA-15 was shown to interact with extracellular signal regulated kinase (ERK, 6) and regulate the nuclear entry of this protein, and several other important interactions with other proteins involved in regulation of apoptosis, glucose metabolism and cell growth have been described (reviewed in 7).
MCA-4D2 was made against a recombinant full length PEA-15 construct expressed in and purified from E. coli. It works on western blots and IF/ICC on a wide range of species but is not recommended for IHC. We also supply an alternate mouse monoclonal antibody to PEA-15 MCA-4D117 which can be used for IHC. Mouse select image above left for larger view.
1. Araujo, H. et al. Characterization of PEA-15, a major substrate for protein kinase C in astrocytes. J. Biol. Chem. 268:5911-20 (1993).
2. Estelles, A. et al. The Major Astrocytic Phosphoprotein PEA-15 Is Encoded by Two mRNAs Conserved on Their Full Length in Mouse and Human. J. Biol. Chem. 271:14800-6 (1996).
3. Condorelli, G. et al. PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitusEMBO J. 17:3858-66 (1998).
4. Sharif, A. et al. The expression of PEA-15 (phosphoprotein enriched in astrocytes of 15 kDa) defines subpopulations of astrocytes and neurons throughout the adult mouse brain. Neurosci. 126:263-75 (2004).
5. Boldin, MP. et al. A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain. J. Biol. Chem. 271:7795–78 (1995).
6. Ramos, JW. et al. PEA-15 Mediates Cytoplasmic Sequestration of ERK MAP Kinase. Dev. Cell 1:239–50 (2001).
7. Fiory, F. et al. Frontiers: PED/PEA-15, a multifunctional protein controlling cell survival and glucose metabolism. Am. J. Physiol. Endocrinol. Metab. 297:E592-601 (2009).
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Contact info
EnCor Biotechnology Inc.
4949 SW 41st Boulevard, Ste 40
Gainesville
Florida 32608 USA
Phone: (352) 372 7022
Fax: (352) 372 7066
E-mail: admin@encorbio.com