| Name: | Mouse monoclonal antibody to human annexin A6 |
| Immunogen: | Recombinant full length annexin A6 expressed in and purified from E. coli |
| HGNC Name: | ANXA6 |
| UniProt: | P08133 |
| Molecular Weight: | 75kDa, ~68kDa by SDS-PAGE |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Human, rat, mouse, dog, horse, monkey |
| RRID: | AB_2923497 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, ICC/IF |
| Recommended Dilutions: | WB: 1:1,000-1:2,000. ICC/IF: 1:1,000. IHC not recommended |
| Storage: | Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, MCA-4G3, dilution 1:500 in red, and costained with chicken pAb to vimentin, CPCA-Vim, dilution 1:2,000 in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.
Western blot analysis of different cell lysates using mouse mAb to annexin A6, MCA-4G3, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to RPCA-HSP60, dilution 1:5,000, in red. The band at 60kDa corresponds to HSP60 protein.
Mouse Monoclonal Antibody to Annexin A6
Cat# MCA-4G3
$120.00 – $800.00
The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner (1). Different members of the family were discovered by different laboratories and as a result the various members have many alternate names, such as lipocortin, calpactin, calelectrin and very many others. In fact Annexin A6 has a particularly surprising number of alternate names, 19 being listed on the Genecards ANXA6 site. The widely used current nomenclature is now based on a letter to indicate membership in a particular one of several annexin sub-families and a number for individual gene products, hence the name annexin A6. The annexin family is defined by a compact disc structure formed from 16 closely packed α-helices which co-ordinate multiple Calcium ions with phospholipid containing membranes. This domain is defined by 4 imperfect repeats of a ~77 amino acid sequence, each repeat forming 4 α-helices (2,3). Annexin A6 was first cloned and sequenced as p68, as a Calcium dependent membrane binding protein extracted from B lymphoblastoid cells (4) and independently as the human homolog of calelectrin, a protein isolated from the electric organ of the fish Torpedo (5). The annexin A6 protein sequence was found to be 75kDa in molecular size, about twice the size of most other annexin family members, and proved to have two of the 16 α-helical regions apparently generated by duplication of the annexin core. The protein has frequently been reported to run on SDS-PAGE more rapidly that expected from the expected 75kDa, at ~68kDa, likely related to the high content of negatively charged amino acids (5). Annexin A6 is normally localized in the cytosol but becomes membrane associated following cellular injury, and so is often seen in ““blebs”, transient herniations of the plasma membrane associated with apoptosis and more generally indicative of cell stress. Recent studies suggest that annexin A6 functions, in combination with annexin A4, in the repair of cell membranes following cellular injury (6).
The MCA-4G3 antibody was made against full length recombinant human annexin A6 expressed in and purified from E. coli. The antibody is recognizes annexin A6 in human, rodents and many other mammals and can be used as a marker of blebs, indicative of cellular stress. We do not recommend the use of this antibody for IHC. e also market a rabbit polyclonal antibody to annexin A6 RPCA-ANXA6. Mouse select image above left for larger view.
br>
1. Gerke V, and Moss SE. Annexins: from structure to function. Physiol Revs 82:331-71 (2002).
2. Barton GJ. et al. Amino acid sequence analysis of the annexin super-gene family of proteins. Eur J Biochem 198:749-60 (1991).
3. Geisow MJ. et al. A consensus amino-acid sequence repeat in Torpedo and mammalian Ca2+-dependent membrane-binding proteins. Nature 320:636-8 (1986).
4, Crompton MR et al. Primary structure of the human, membrane-associated Ca2+-binding protein p68: a novel member of a protein family. EMBO J 7:21-27 (1988).
5. Suedhof TC et al. Human 67-kDa calelectrin contains a duplication of four repeats found in 35-kDa lipocortins. PNAS 85:664-668 (1988).
6. Boye TL et al. Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. Nature Comm 8:1623 (2017).
