| Name: | Mouse Monoclonal Antibody to DJ1 |
| Immunogen: | Full length recombinant human DJ1 expressed in and purified from E. coli. |
| HGNC Name: | PARK7 |
| UniProt: | Q99497 |
| Molecular Weight: | 21kDa |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Human, Cow, no reactivity with rat or mouse |
| RRID: | AB_2572260 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, ICC/IF, IHC |
| Recommended Dilutions: | WB: 1:5,000. IF/IHC: 1:1,000 |
| Storage: | Store at 4°C for short term, for longer term at -20°C |
Immunoflourescent analysis of HeLa cells stained with mouse mAb to DJ1/Park7, MCA-4H4, dilution 1:1,000 in green, and costained with chicken pAb to lamin A/C, CPCA-LaminAC, dilution 1:5,000 in red. MCA-4H4 antibody reveals strong cytoplasmic staining for DJ1 protein, while CPCA-LaminAC antibody produces strong staining of nuclear lamina.
Western blot analysis of whole brain and cell lysates using mouse mAb against DJ1, MCA-4H4, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The MCA-DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, CPCA-Vim, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.
Mouse Monoclonal Antibody to DJ1/PARK7
Cat# MCA-4H4
$120.00 – $800.00
DJ1, also known as PARK7, is a cytoplasmic protein that belongs to the DJ1/ThiJ/PfpI superfamily of proteins, which have a variety of functions in eukaryotes and bacteria (1). DJ1 was originally cloned as an oncogene that cooperatively transforms cells together with mutated H-ras (2). Mutations were then found in the DJ1 gene which were associated with rare forms of autosomal recessive early-onset Parkinson’s disease, which led to DJ1 also being known as PARK7 (3). The function or functions of DJ1 are not well understood may include a redox-sensitive chaperone, a sensor for oxidative stress, and it may protect neurons against oxidative stress and cell death (4-6). Augmenting DJ1 activity might be a novel approach to treating chronic neurodegenerative illnesses such as Parkinson’s disease and acute damage such as stroke (7).
The MCA-4H4 antibody was generated against full length recombinant human DJ1 expressed in and purified from E. coli. This antibody binds to human but not rodent DJ1. The rodent proteins are about 91% identical to the human, a relatively low degree of conservation. The antibody works well for western blotting and for IF, ICC and IHC (see data under “Additional Info” tab). We also supply a rabbit polyclonal antibody to DJ1 RPCA-DJ1 which was also raised against the recombinant human DJ1 but which does bind the rodent DJ1 homologue. While RPCA-DJ1 has general utility for studies of DJ1, MCA-4H4 can be used to identify transplanted human cells or transgenic expression of human DJ1 in rodents. . Mouse select image above left for larger view.
Chromogenic immunostaining of a formalin fixed paraffin embedded human hippocampus section with mouse mAb to DJ1/Park7, MCA-4H4, dilution 1:2,000, detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In hippocampus, MCA-4H4 labels the cytoplasm of glial cells and to a lesser extent neuronal cells. This antibody performs well in staining with NBF fixed human tissues but does not cross react with rodent. Mouse select image for larger view.
1. Bandyopadhyay S, Cookson MR. Evolutionary and functional relationships within the DJ1 superfamily. BMC Evol. Biol. 4:6 (2004).
1. Nagakubo D, et al. DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. BBRC 231:509–13 (1997).
2. Bonifati V, et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science 299:256–9 (2003).
3. Xu J, et al. The Parkinson’s disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum. Mol. Genet. 14:1231-41 (2005).
4. Yokota T, et al. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. BBRC 312:1342–8 (2003).
5. Taira T, et al. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep. 5:213–8 (2004).
6. Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson’s disease. J. Mol. Med. 82:163-74 (2004).
7. Aleyasin H, et al. The Parkinson’s disease gene DJ-1 is also a key regulator of stroke-induced damage. PNAS 104:18748-53 (2007).
