Name: | Mouse monoclonal antibody against C3 anaphylotoxin-like domain |
Immunogen: | Recombinant human C3 N-terminal anaphylatoxin construct, amino acids 668-741 of NP_000055.2 |
HGNC Name: | C3 |
UniProt: | |
Molecular Weight: | 185kDa |
Host: | Mouse |
Isotype: | IgM |
Species Cross-Reactivity: | Human |
RRID: | AB_2572254 |
Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
Applications: | WB, ELISA |
Recommended Dilutions: | WB: 1:5,000-1:10,000 |
Storage: | Store at 4°C for short term, for longer term at -20°C |
Mouse Monoclonal Antibody to Complement C3 α-chain
Cat# MCA-6B1
$120.00 – $800.00
Complement component 3, often simply called C3, is the third protein in the complement system. The complement system “complements” the ability of antibodies and phagocytic cells to clear pathogens such as bacteria and viruses from the organism and can trigger inflammation and remove debris from cells and tissues. C3 is involved in both the classical and alternative pathway. The MCA-6B1 antibody binds to the anaphylotoxin-like domain in the α subunit of human C3.
Complement component 3, often simply called C3, is the third protein in the complement system. The complement system is a part of the immune system to “complement” the ability of antibodies and phagocytic cells to clear pathogens such as bacteria and viruses from the organism, trigger inflammation and remove debris from cells and tissues (1). C3 plays a central role in complement activation, and is involved in both the classical and alternative pathway. C3 is synthesized as an intracellular precursor (pro-C3) of 185 kDa which is processed by proteolytic cleavage into two large chains called the α subunit (115 kDa) and the β sunbunit (70 kDa) which are however linked by a disulfide bond (2).
C3 activation involves further cleavage by C3 convertase to produce C3a (9 kDa) and C3b (the remaining 176 kDa of the β subunit and truncated α subunits). C3a is released into the surrounding fluids and can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin, and the N-terminal region of the α subunit is referred to as the anaphyloxin domain. In blood, C3a may be further cleaved by carboxypeptidase to produce C3adesArg or ASP (acylation-stimulating protein), which acts as a paracrine signal to increase triglyceride synthesis in adipocytes (3). C3adesArg have been demonstrated to be present at increased levels in patients with obesity, diabetes mellitus type 2 and coronary artery disease (4,5,6). C3b is the main effector molecule of the complement system, expressing multiple binding sites for other complement components such as C5, properdin, factor B, factor H and certain membrane proteins such as MCP). Binding these proteins to C3b leads either to amplification of C3 convertase, or initiation of Membrane Attack Complex (MAC). C3b also serves as an opsonizing agent to bind to the pathogen and target it for destruction by phagocytes. On the other hand, binding of C3b to complement component, factor I and a co-factor, inactivates C3b to iC3b and release C3f (2 kDa). iC3b can further be cleaved to form C3c, C3dg which further produces C3d and C3g (3). Overall, C3 promotes phagocytosis, supports local inflammatory responses against pathogens, and instructs the adaptive immune response to select the appropriate antigens for a humoral response (6). More recently, C3 has been suggested to have a pathophysiological role in Alzheimer’s and other neurodegenerative disorders (7). In clinical practice the level of (C3) in serum and CSF can be used to help identify immunological disorders, especially those associated with deficiencies of complement components.
Additional References
6. Cianflone K, Zhang XJ, Genest J Jr, Sniderman A. Plasma acylation-stimulating protein in coronary artery disease.Arterioscler Thromb Vasc Biol.17:1239-44 (1997).
7. Wang Y, Hancock AM, Bradner J, Chung KA, Quinn JF, Peskind ER, Galasko D, Jankovic J, Zabetian CP, Kim HM, Leverenz JB, Montine TJ, Ginghina C, Edwards KL, Snapinn KW, Goldstein DS, Shi M, Zhang J. Complement 3 and Factor H in Human Cerebrospinal Fluid in Parkinson’s Disease, Alzheimer’s Disease, and Multiple-System Atrophy. The American Journal of Pathology, Vol. 178:1509-16 (2011).
1. Janeway CA et al. The complement system and innate immunity. The Immune System in Health and Disease. 5th edition (2001).
2. Brade V, Hall RE, Colten HR. Biosynthesis of pro-C3, a precursor of the third component of complement.J. Exp. Med. 146:759-765 (1977).
3. Xia Z, ET AL. Acylation-stimulating Protein (ASP)/Complement C3adesArg Deficiency Results in Increased Energy Expenditure in Mice. J. Biol. Chem. 279:4051-7 (2004).
4. Maslowska M, et al. Plasma acylation stimulating protein, adipsin and lipids in non-obese and obese populations. Eur. J. Clin. Inv. 29:679–86 (1999).
5. Koistinen HA, et al. Plasma Acylation Stimulating Protein Concentration and Subcutaneous Adipose Tissue C3 mRNA Expression in Nondiabetic and Type 2 Diabetic Men. Arteriosclerosis, Thrombosis, and Vascular Biology 21:1034–9 (2001).
Related products
-
Mouse Monoclonal Antibody to Human Ki67, Ki-67
$120.00 – $800.00
Cat# MCA-6B4Select options This product has multiple variants. The options may be chosen on the product page -
Mouse Monoclonal Antibody to Peripherin
$120.00 – $800.00
Cat# MCA-7C5Select options This product has multiple variants. The options may be chosen on the product page -
Mouse Monoclonal Antibody to Pdi1p
$120.00 – $800.00
Cat# MCA-38H8Select options This product has multiple variants. The options may be chosen on the product page -
Mouse Monoclonal Antibody to DJ1/PARK7
$120.00 – $800.00
Cat# MCA-4H4Select options This product has multiple variants. The options may be chosen on the product page
Contact info
EnCor Biotechnology Inc.
4949 SW 41st Boulevard, Ste 40
Gainesville
Florida 32608 USA
Phone: (352) 372 7022
Fax: (352) 372 7066
E-mail: admin@encorbio.com