| Name: | Mouse monoclonal antibody to peripherin, rodent specific |
| Immunogen: | Recombinant rat peripherin purified from E. coli |
| HGNC Name: | PRPH |
| UniProt: | P41219 |
| Molecular Weight: | ~57kDa |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Rat, Mouse, not Human, Pig, Cow |
| RRID: | AB_2572374 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC of rodent tissue |
| Recommended Dilutions: | WB: 1:1,000. IF/ICC: 1:200 IHC: 1:1,000 |
| Storage: | Stable at 4°C for one year, for longer term store at -20°C |
Immunofluorescent analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with mouse mAb to peripherin, MCA-7C5, dilution 1:500 in red, and costained with chicken pAb to vimentin, CPCA-Vim, dilution 1:10,000, in green. The blue is Hoechst staining of nuclear DNA. The MCA-7C5 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.
Western blot analysis of tissue and cell lysates using mouse mAb to peripherin, MCA-7C5, dilution 1:1,000 in green: [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.
Mouse Monoclonal Antibody to Peripherin
Cat# MCA-7C5
$120.00 – $800.00
Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name (1). Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also as a major cytoskeletal protein in rat pheochromacytoma PC12 cells (2,3). Peripherin is expressed in certain neuroendocrine cells and in the insulin producing cells of the pancreas, and autoantibodies to peripherin may be seen in diabetes mellitus (4). It is also prominently expressed in certain tumors and in the ballooned axons typical of amyotrophic lateral sclerosis (5,6). Different classes of neuron and nerve fiber may be identified based on their expression of peripherin, NF-L and other markers (e.g. 7). There are multiple transcripts from the single peripherin gene, some of which encode proteins which produce proteins unable to form intermediate filaments and which induce the formation of pathological inclusions (8,9). Note that the intermediate filament subunit peripherin (HGNC PRPH) is completely unrelated to peripherin/RDS (HGNC PRPH2), a retinal protein belonging to the tetraspanin family.
The MCA-7C5 antibody was made against full length recombinant rat peripherin expressed in and purified from E. coli. It recognizes rat and mouse peripherin but does not recognize the human, pig or bovine proteins. The antibody works well for western blotting and for IF, ICC and IHC on rodent material (for IHC see data under “Additional Info” tab). We also manufacture a mouse monoclonal antibody to peripherin which has been used in studies of peripherin expression for many years and in many peer reviewed publications, MCA-8G2 which recognizes peripherin in human, rodent and other species. We also made rabbit and chicken polyclonal antibodies to peripherin, RPCA-Peri, and CPCA-Peri. Mouse select image above left for larger view.
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat brain stem section with mouse mAb to peripherin, MCA-7C5, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In brain, MCA-7C5 labels the cytoplasm of a specific subset of neurons within the brain stem expected to have projections towards the periphery. This antibody performs well in testing with 4% PFA fixed tissues but was not tested for immunohistochemistry on NBF fixed paraffin sections.MCA-7C5 is our recommended clone for immunostaining rat tissue. It does not cross react with the human protein. Mouse select image for larger view.
1. Portier MM, de Néchaud B, Gros F. Peripherin, a new member of the intermediate filament protein family. Dev. Neurosci. 6:335-44 (1984).
2. Troy CM, Brown K, Greene LA, Shelanski ML. Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo. Neuroscience 36:217-37 (1990).
3. Aletta JM, et al. Relationship between the nerve growth factor-regulated clone 73 gene product and the 58-kilodalton neuronal intermediate filament protein (peripherin). J. Neurochem 51:1317-20 (1988).
4. Puertas MC, et al. Peripherin Is a Relevant Neuroendocrine Autoantigen Recognized by Islet-Infiltrating B Lymphocytes. J. Immunol. 178:6533-9 (2007).
5. Migheli A, et al. Peripherin immunoreactive structures in amyotrophic lateral sclerosis. Lab. Invest. 68:185-91 (1993).
6. He CZ, Hays AP. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54 (2004).
7. Goldstein ME, House SB, Gainer H. NF‐L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells. J. Neurosci. Res. 30:92-104 (1991).
8. Landon F, et al. Multiple mRNAs encode peripherin, a neuronal intermediate filament protein. EMBO J. 8:1719-26 (1989).
9. Robertson J, et al. A neurotoxic peripherin splice variant in a mouse model of ALS. J. Cell Biol. 60:939-49 (2003).
