| Name: | Rabbit polyclonal antibody to tyrosine hydroxylase |
| Immunogen: | Full length human TH expressed in and purified from E. coli. |
| HGNC Name: | TH |
| UniProt: | P07101 |
| Molecular Weight: | ~58kDa |
| Host: | Rabbit |
| Isotype: | |
| Species Cross-Reactivity: | Human, rat, mouse |
| RRID: | AB_2737417 |
| Format: | Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC |
| Recommended Dilutions: | WB: 1:5,000. IF/ICC 1:10,000 |
| Storage: | Stable at 4°C for one year, for longer term store at -20°C |
Immunofluorescent analysis of rat brain section stained with rabbit pAb to tyrosine hydroxylase, RPCA-TH, dilution 1:10,000, in red and costained with mouse mAb to pNF-H, MCA-AH1, dilution 1:1,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The RPCA-TH antibody stains the striatal TH expressing interneurons, while the pNF-H antibody labels axons from other neuronal cells.
Western blot analysis of different tissue and cell lysates using rabbit pAb to TH, RPCA-TH, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] SH-SY5Y cells, and [5] PC12 cells. The strong band at about 60kDa corresponds to TH protein expected in brain and PC12 extracts but not in SH-SY5Y cells.
Rabbit Polyclonal Antibody to Tyrosine Hydroxylase
RPCA-TH
$150.00 – $1,000.00
Tyrosine hydroxylase (TH) is a vital enzyme responsible for the generation of L-DOPA from the amino acid tyrosine. L-DOPA is the direct precursor of the neurotransmitter dopamine, and dopamine can itself be processed to produce the neurotransmitters adrenalin and noradrenalin (a.k.a. epinephrin and norepinephrin respectively). Neurons which use dopamine, adrenalin or noradrenaline, called collectively chatecholamines, must express TH. TH has a very restricted distribution in the brain but is highly expressed in the cells in which it is found. As a result antibodies to TH are useful for the identification of chatecholaminergic neurons. TH positive neurons in the rat are localized into clusters of cells most of which are in the brain stem, including notably the substantia nigra and locus ceruleus (1,2). The clusters of cells are usually referred to by a classification scheme based on that proposed by Dahlstrӧm and Fuxe, which labels cells in groups A1 – A17 and C1 to C3 (2). Subpopulations of neurons are localized in the olfactory bulb, habenula and retina. TH positive cells are also found in a subset of cells in the adrenal medulla, sympathetic ganglia, sensory ganglia and enteric ganglia (2). Numerous TH positive axons can be seen coursing through the striatum and to a much lesser degree the cortex originating from the mid brain A8, A9 and A10 nuclei. TH neurons have a huge impact on brain function and behavior but are relatively infrequent- the rat brain contains about 22,000 TH positive neurons in the A8, A9 and A10 nuclei out of a total of 200 million neurons (3). Parkinson’s disease is caused by the loss of TH positive dopaminergic neurons in the substantia nigra, which are also relatively low in number (4), and perturbation of TH neurons has been implicated in Alzheimer’s disease and schizophrenia (5-7). There is one mammalian gene which produces one mRNA transcript and one protein in rat but four alternate mRNA transcripts produce four slightly different forms of TH proteins in humans (8).
RPCA-TH was made against full length recombinant human TH based on the 524 amino acid sequence in NP_954987.2, expressed in and purified from E. coli. The antibody works well on cells in culture and tissue sections, including formalin fixed paraffin embedded human and rodent tissues, see data under the “additional info” tag. We also supply a mouse monoclonal and a rabbit polyclonal antibody to this protein, MCA-4H2 and CPCA-TH. Mouse select image at left for larger view.
Chromogenic immunostaining of a NBF fixed paraffin embedded human midbrain section with rabbit pAb to tyrosine hydroxylase, RPCA-TH, dilution 1:10,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, RPCA-TH antibody labels dopaminergic neurons and their axons traversing the striatum. This antibody performs well in testing with 4% PFA and standard NBF fixed mouse, rat and human tissue. Mouse select image for larger view.
1. Pickel VM, et al. Cellular localization of tyrosine hydroxylase by immunohistochemistry. J. Histochem. Cytochem. 23:1-12 (1975).
2. Bjorklund A, Dunnett SB. Dopamine neuron systems in the brain: an update. Trends Neurosci. 30:194-202 (2007).
3. German DC, Manaye KF. Midbrain dopaminergic neurons (nuclei A8, A9, and A10): three-dimensional reconstruction in the rat. J. Comp. Neurol. 331:297-309 (1993).
4. Daubner SC, Le T, Wang S. Tyrosine hydroxylase and regulation of dopamine synthesis. Arch. Biochem. Biophys. 508:1-12 (2011).
5. Haavik J, Toska K. Tyrosine hydroxylase and Parkinson’s disease. Mol. Neurobiol. 16:285-309 (1988).
6. Torack RM, Morris C. Tyrosine hydroxylase-like (TH) immunoreactivity in Parkinson’s disease and Alzheimer’s disease. J. Neural Transm. Park. Dis. Dement. Sect. 4:165-71 (1992).
7. Benes FM, Todtenkopf MS, Taylor JB. Differential distribution of tyrosine hydroxylase fibers on small and large neurons in layer II of anterior cingulate cortex of schizophrenic brain. Synapse 25:80-92 (1997).
8. Lewis DA, Melchitzky DS, Haycock JW. Four isoforms of tyrosine hydroxylase are expressed in human brain. Neuroscience 54:477-92 (1993)
