| Name: | Rabbit polyclonal to Neurofilament Light, NF-L |
| Immunogen: | Recombinant full length human NF-L protein |
| HGNC Name: | NEFL |
| UniProt: | P07196 |
| Molecular Weight: | 68-70kDa |
| Host: | Rabbit |
| Isotype: | |
| Species Cross-Reactivity: | Human, Rat, Mouse, Cow, Pig |
| RRID: | AB_2572364 |
| Format: | Supplied as an aliquot of serum plus 5mM NaN3 |
| Applications: | WB IF/ICC |
| Recommended Dilutions: | WB: 1: 10,000-1:15,000. IF/ICC: 1:5,000. |
| Storage: | Store at 4°C for short term, for longer term, store at -20°C |
Immunofluorescent analysis of mouse cerebellum section stained with rabbit pAb to NF-L, RPCA-NF-L, dilution 1:5,000 in red, and costained with chicken pAb to MBP, CPCA-MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. RPCA-NF-L antibody labels dendrites and axons of neuronal cells, and MBP antibody stains myelin sheathes around axons.
Western blot analysis of different tissue lysates using rabbit pAb to NF-L, RPCA-NF-L, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.
Rabbit Polyclonal Antibody to Neurofilament NF-L
Cat# RPCA-NF-L
$120.00 – $800.00
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like MCA-DA2 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others (2-4). Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration (5).
RPCA-NF-L antibody was made against a preparation of recombinant full length human NF-L and binds NF-L from a variety of mammalian species including human, rat and mouse. We also generated a highly specific chicken polyclonal antibody, CPCA-NF-L, a rabbit polyclonal antibody to the C-terminal peptide of rat NF-L protein, RPCA-NF-L-ct and mouse monoclonal antibodies, MCA-DA2, MCA-7D1, MCA-1B11, and MCA-6H112. Mouse select image at left for larger view.
Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with rabbit pAb to NF-L, RPCA-NF-L, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, RPCA-NF-L labels the perinkarya and dendrites of Purkinje cells and the projections of neuronal cells within the granular layer. This antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse and human tissues. Mouse select image for larger view.
1. Hoffman et al. Neurofilament gene expression:a major determinant of axonal caliber. PNAS 84:3472-6 (1987).
2. Perrot R, et al. Review of the Multiple Aspects of Neurofilament Functions, and their Possible Contribution to Neurodegeneration. Mol. Neurobiol. 38:27-65 (2008).
3. Lépinoux-Chambaud C. Eyer J. Review on intermediate filaments of the nervous system and their pathological alterations. Histochem. Cell Biol. 140:13-22 (2013).
4. Liu Q. et al. Neurofilamentopathy in Neurodegenerative Diseases. Open Neurol. J. 5:58–62 (2011).
5. Bacioglu M, et al. Neurofilament light chain in blood and CSF as marker of disease progression in mouse models and in neurodegenerative diseases. Neuron 91:56-66 (2016).
