| Name: | Rabbit polyclonal antibody to human beta-synuclein |
| Immunogen: | Full length human recombinant β-Synuclein protein expressed in and purified from E. Coli |
| HGNC Name: | SNCB |
| UniProt: | Q16143 |
| Molecular Weight: | ~15kDa |
| Host: | Rabbit |
| Isotype: | |
| Species Cross-Reactivity: | Human, Rat, Mouse, Cow, Pig |
| RRID: | AB_2860578 |
| Format: | Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC |
| Recommended Dilutions: | WB: 1:1,000-2,000. IF/ICC 1:1,000-2,000. IHC 1:2,000. |
| Storage: | Store at 4°C for short term, for longer term store at -20°C |
Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to β-synuclein, RPCA-SNCB, dilution 1:1,000 in red, and costained with chicken pAb to parvalbumin, CPCA-Pvalb, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The β-synuclein antibody detects protein concentrated in synaptic regions, and parvalbumin antibody labels the perikarya and dendrites of Purkinje cells, and interneurons in the molecular layer of the cerebellum.
Western blot analysis of different tissue lysates using rabbit pAb to β-synuclein, RPCA-SNCB, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse cerebellum [3] mouse hippocampus, [4] rat cerebellum, [5] rat hippocampus, and [6] cow cerebellum. Strong band at about 17kDa corresponds to the β-synuclein protein.
Rabbit Polyclonal Antibody to β-synuclein
RPCA-SNCB
$120.00 – $800.00
β-synuclein is a member of the synuclein protein family, the other two members being α and γ-synuclein, each protein being coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo (1), and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, so that it is a major component of the Lewy bodies of Parkinson’s disease (2-5). β-synuclein was isolated as a component of normal and diseased human brain as a protein clearly related to but distinct from α-synuclein (6). The human β-synuclein molecule is 134 amino acids in size compared to 140 amino acids for α-synuclein, and the N-terminal halves of the two molecules are virtually identical while the C-terminal regions is more variable. As a result we made our new β-synuclein antibodies to this region. Like α-synuclein, β-synuclein is heavily concentrated in the brain in presynaptic regions. A third synuclein, γ-synuclein was originally identified as breast cancer specific gene 1, (BCSG1), but is also heavily expressed in brain and also has a similar N-terminal sequence (7). The three synucleins appear to have overlapping functions so genetic deletion of all three in mice is required to obtain serious neurological deficits (7).
The RPCA-SNCB antibody was made against full length human recombinant β-synuclein protein expressed in and purified from E.Coli. This antibody recognizes full length human and rodent β-synuclein specifically both in western blots and in immunocytochemical experiments. EnCor also supplies a high quality monoclonal antibody to β-synuclein MCA-6A10, and high quality antibodies to α-synuclein MCA-2A7 and CPCA-SCNA. Mouse select image at left for larger view.
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded mouse hippocampus section with rabbit pAb to β-synuclein, RPCA-SNCB, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. β-synuclein is abundant in pre-synaptic regions of the brain. This antibody performs well in testing with both 4% PFA and standard NBF fixed mouse, human, and rat tissues. Mouse select image for larger view.
1. Maroteaux L, Campanelli JT, Scheller RH. Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal. J. Neurosci. 8:2804-15 (1988).
2. Lavedan C. The Synuclein Family. Genome Research 8:871-80 (1998).
3. Polymeropoulos, MH et al. Mutation in the alpha-synuclein gene identified in families with Parkinson’s disease. Science 276:2045-7 (1997).
4. Kruger, R et al. Ala30-to-Pro mutation in the gene encoding alpha-synuclein in Parkinson’s disease. Nature Genet. 18:106-8 (1998).
5. Chartier-Harlin, M-C. et al. Alpha-synuclein locus duplication as a cause of familial Parkinson’s disease. Lancet 364:1167-9 (2004).
6. Ji H. et al. Identification of a breast cancer-specific gene, BCSG1, by direct differential cDNA sequencing. Cancer Res. 57:759-64 (1997).
7. Greten-Harrison, B. et al. αβγ-Synuclein triple knockout mice reveal age-dependent neuronal dysfunction. PNAS 107:19573-8 (2001).
