| Name: | Rabbit Polyclonal to Vimentin |
| Immunogen: | Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. expressed in and purified from E. coli. |
| HGNC Name: | VIM |
| UniProt: | P08670 |
| Molecular Weight: | 50kDa |
| Host: | Rabbit |
| Isotype: | |
| Species Cross-Reactivity: | Human, rat, mouse, cow, pig, horse |
| RRID: | AB_2572398 |
| Format: | Antibody is supplied as an aliquot of serum plus 5mM NaN3 |
| Applications: | Western blot, ICC/IF, IHC |
| Recommended Dilutions: | Western blot: 1:10,000. IF/ICC and IHC: 1:5,000. |
| Storage: | Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:5,000, in green, and mouse mAb to Actin, MCA-5J11, dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RPCA-Vim, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.
Rabbit Polyclonal Antibody to Vimentin
Cat# RPCA-Vim
$120.00 – $800.00
Vimentin is a protein subunit of the intermediate or 10nm filaments found in the cytoplasm of many cell types (1). Intermediate filaments are relatively stable fibrous components of cells which appear to have primarily a mechanical function. Many cell lines such as HEK293, HeLa, 3T3 and Cos cells contain prominent vimentin networks (1). Vimentin is a major protein of eye lens and cornea, and found generally in mesenchymal tissues in adult mammals. In the CNS it is found in endothelia and developing neurons, developing and some mature astrocytes, microglia, mature Bergmann glia and ependyma (2,3). Mutations in the vimentin gene may cause cataracts (4,5), and elevated levels of vimentin in blood samples are associated with onset of cancer (6,7). Vimentin levels increase in a variety of cell types as they become cancerous, suggesting that increase in expression of this protein is a useful diagnostic marker of the epithelial-mesenchymal transition (8).
Antibodies to vimentin are useful in studies of stem cells and generally to reveal the intermediate filament cytoskeleton. The immunogen used to generate this antibody was full length recombinant human vimentin, PROT-r-Vim, expressed in and purified from E. coli. The antibody works well on all mammals tested to date on western blots and cells in culture and in sections. The same vimentin immunogen was used to produce two high quality epitope mapped monoclonal antibodies to vimentin MCA-2A52 and MCA-2D1, and also a popular chicken polyclonal antibody CPCA-VIM. Mouse select image at left for larger view.
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded mouse kidney section with rabbit pAb to vimentin, RPCA-Vim, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with Borg buffer retrieval (Biocare Medical). Hematoxylin (blue) was used as the counterstain. In this image, the RPCA-Vim antibody specifically labels the interstitium in glomerular podocytes. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues with high pH, EDTA based retrieval. Mouse select image for larger view.
1. Franke WW, et al. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. PNAS 75:5034–8 (1978).
2. Dahl D, et al. Vimentin, the 57 000 molecular weight protein of fibroblast filaments, is the major cytoskeletal component in immature glia. Eur. J. Cell Biol. 24:191-6 (1981).
3. Shaw, G. et al. An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain. Eur. J. Cell Biol. 26:68-72 (1981).
4. Muller M, et al. Dominant cataract formation in association with a vimentin assembly disrupting mutation. Hum. Molec. Genet. 18:1052-7 (2009).
5. Zhai Y, et al. Targeted exome sequencing of congenital cataracts related genes: broadening the mutation spectrum and genotype-phenotype correlations in 27 Chinese Han families. Sci. Rep. 7:1219 (2017).
6. Satelli A, Li S. Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol. Life Sci. 68:3033-46 (2011).
7. Wong KF, Luk JM. Discovery of lamin B1 and vimentin as circulating biomarkers for early hepatocellular carcinoma. Meth. Mol. Biol. 2909:295-310 (2012).
8. Jia X, et al. Vimentin-a potential biomarker for therapeutic efficiency of HAART. Acta Biochim. Biophys. Sin. (Shanghai) 6:1001-6 (2014).
